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<p>A novel bacterium, strain SANA<sup>T</sup>, was isolated from a xenic culture of a bacterivorous protist which was obtained from the sulphidogenic bottom sediment of saline Lake Hiruga in Fukui, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain SANA<sup>T</sup> clustered with members of the family <i>Gottschalkiaceae</i>. The closest relative of the strain SANA<sup>T</sup> was <i>Andreesenia angusta</i> MK-1<sup>&#xa0;T</sup> with 16S rRNA gene sequence identity of 91.75%, suggesting that SANA<sup>T</sup> is a novel genus. Values of digital DNA-DNA hybridisation (&lt; 29.7%) and average nucleotide identity (&lt; 67.6%) between strain SANA<sup>T</sup> and species in the families <i>Gottschalkiaceae</i> and <i>Tissierellaceae</i> were lower than threshold for species delineation. Cells were strictly anaerobic, non-motile rods and gave a negative Gram-stain reaction. Cells first elongated, and then the elongated cells were divided in the presence of either serum components, glucose, fructose or maltose but not uric acid. Na<sup>+</sup> was required to grow SANA<sup>T</sup>. Growth was observed at pH 6.0–9.0 and at temperature between 10 and 25&#xa0;°C. The major cellular fatty acids were C<sub>16:1</sub> <i>cis</i> 9, C<sub>14:0</sub>, C<sub>16:0</sub>, C<sub>16:0</sub> DMA and summed feature 6 (C<sub>16:1</sub> <i>cis</i> 7 DMA or anteiso-C<sub>15:0</sub> 3-OH). The genomic G + C content of strain SANA<sup>T</sup> was 42.1%. Based on the genetic and phenotypic features, strain SANA<sup>T</sup> represents a novel species of a novel genus within the novel family <i>Kamagatellaceae</i> fam. nov., for which the name <i>Kamagatella elastica</i> gen. nov., sp. nov. is proposed, with the type strain SANA<sup>T</sup> (= DSM 120061<sup>&#xa0;T</sup> = JCM 39611<sup>&#xa0;T</sup>).</p>

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Kamagatella elastica gen. nov., sp. nov., an anaerobic bacterium representing the family Kamagatellaceae fam. nov. within the order Tissierellales, isolated from a xenic culture of an anaerobic bacterivorous protist

  • 期刊论文

  • 发表日期：

    2026年4月10日

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  • Ryuji Kondo,
  • Takafumi Kataoka

摘要

A novel bacterium, strain SANAT, was isolated from a xenic culture of a bacterivorous protist which was obtained from the sulphidogenic bottom sediment of saline Lake Hiruga in Fukui, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain SANAT clustered with members of the family Gottschalkiaceae. The closest relative of the strain SANAT was Andreesenia angusta MK-1 T with 16S rRNA gene sequence identity of 91.75%, suggesting that SANAT is a novel genus. Values of digital DNA-DNA hybridisation (< 29.7%) and average nucleotide identity (< 67.6%) between strain SANAT and species in the families Gottschalkiaceae and Tissierellaceae were lower than threshold for species delineation. Cells were strictly anaerobic, non-motile rods and gave a negative Gram-stain reaction. Cells first elongated, and then the elongated cells were divided in the presence of either serum components, glucose, fructose or maltose but not uric acid. Na+ was required to grow SANAT. Growth was observed at pH 6.0–9.0 and at temperature between 10 and 25 °C. The major cellular fatty acids were C16:1 cis 9, C14:0, C16:0, C16:0 DMA and summed feature 6 (C16:1 cis 7 DMA or anteiso-C15:0 3-OH). The genomic G + C content of strain SANAT was 42.1%. Based on the genetic and phenotypic features, strain SANAT represents a novel species of a novel genus within the novel family Kamagatellaceae fam. nov., for which the name Kamagatella elastica gen. nov., sp. nov. is proposed, with the type strain SANAT (= DSM 120061 T = JCM 39611 T).