Scholarlink

<p>A novel Gram-stain-negative, aerobic, motile, and rod-shaped bacterium, designated strain 20L07<sup>T</sup>, was isolated from the gut of a larva of the red-brown longhorn beetle (<i>Stictoleptura succedanea</i>). The isolate was catalase-positive and oxidase-negative. Growth of strain 20L07<sup>T</sup> occurred at 10–30&#xa0;°C (optimum, 20–25&#xa0;°C), pH 4–9 (optimum, pH 7), and in the presence of 0–3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 20L07<sup>T</sup> belongs to the genus <i>Dyella</i>, showing the highest sequence similarity with <i>Dyella silvatica</i> RBB189<sup>T</sup> (98.8%), <i>D. soli</i> JS12-10<sup>T</sup> (98.6%), and <i>D. tabacisoli</i> L4-6<sup>T</sup> (98.3%). The genome sequence of strain 20L07<sup>T</sup> comprised 4.6 Mb with a genomic DNA G + C content of 60.5%. The orthologous average nucleotide identity and digital DNA-DNA hybridization values between strain 20L07<sup>T</sup> and <i>D. silvatica</i> RBB189<sup>T</sup> were 75.8% and 21.4%, respectively. Additionally, genome-scale average amino acid identity analysis supported the assignment of strain 20L07<sup>T</sup> to the genus <i>Dyella</i>. The major respiratory quinone was ubiquinone-8 (Q-8). The predominant cellular fatty acids (&gt; 10%) were <i>iso</i>-C<sub>15:0</sub>, <i>iso</i>-C<sub>16:0</sub>, and summed feature 9 (C<sub>16:0</sub> 10-methyl and/or <i>iso</i>-C<sub>17:1</sub> <i>ω</i>9<i>c</i>). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids, and one unidentified aminolipid. In silico analysis of 16S rRNA amplicon datasets using the Integrated Microbial Next Generation Sequencing platform indicated that sequences closely matching strain 20L07ᵀ are prevalent in soil- and insect-associated environments, suggesting its ecological relevance. Based on the phylogenetic, phenotypic, chemotaxonomic, and genomic data, strain 20L07<sup>T</sup> represents a novel species of the genus <i>Dyella</i>, for which the name <i>Dyella stictolepturae</i> sp. nov. is proposed. The type strain is 20L07<sup>T</sup> (= KCTC 8632<sup>T</sup> = JCM 37315<sup>T</sup>).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dyella stictolepturae sp. nov., isolated from the larva gut of red-brown longhorn beetle (Stictoleptura succedanea)

  • 期刊论文

  • 发表日期：

    2026年3月28日

查看全文
  • Bora Kim,
  • Ah-In Yang,
  • Sung-Hong Joe,
  • Hae-In Joe,
  • Na-Ri Shin

摘要

A novel Gram-stain-negative, aerobic, motile, and rod-shaped bacterium, designated strain 20L07T, was isolated from the gut of a larva of the red-brown longhorn beetle (Stictoleptura succedanea). The isolate was catalase-positive and oxidase-negative. Growth of strain 20L07T occurred at 10–30 °C (optimum, 20–25 °C), pH 4–9 (optimum, pH 7), and in the presence of 0–3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 20L07T belongs to the genus Dyella, showing the highest sequence similarity with Dyella silvatica RBB189T (98.8%), D. soli JS12-10T (98.6%), and D. tabacisoli L4-6T (98.3%). The genome sequence of strain 20L07T comprised 4.6 Mb with a genomic DNA G + C content of 60.5%. The orthologous average nucleotide identity and digital DNA-DNA hybridization values between strain 20L07T and D. silvatica RBB189T were 75.8% and 21.4%, respectively. Additionally, genome-scale average amino acid identity analysis supported the assignment of strain 20L07T to the genus Dyella. The major respiratory quinone was ubiquinone-8 (Q-8). The predominant cellular fatty acids (> 10%) were iso-C15:0, iso-C16:0, and summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids, and one unidentified aminolipid. In silico analysis of 16S rRNA amplicon datasets using the Integrated Microbial Next Generation Sequencing platform indicated that sequences closely matching strain 20L07ᵀ are prevalent in soil- and insect-associated environments, suggesting its ecological relevance. Based on the phylogenetic, phenotypic, chemotaxonomic, and genomic data, strain 20L07T represents a novel species of the genus Dyella, for which the name Dyella stictolepturae sp. nov. is proposed. The type strain is 20L07T (= KCTC 8632T = JCM 37315T).