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<p>A Gram-stain-negative, aerobic, and rod-shaped bacterium, designated H14<sup>T</sup>, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. Colonies of strain H14<sup>T</sup> were yellow, round, smooth and convex after cultivating on marine agar at 37 ℃ for 72&#xa0;h. Cells were catalase-positive and oxidase-negative. Growth occurred at 16–40 ℃ (optimum, 37 ℃), pH 5.0–8.5 (optimum, pH 6.5) and with 0.5–10.0% (]/v) NaCl (optimum, 3.0%). Strain H14<sup>T</sup> showed highest 16S rRNA gene sequence similarity of 97.7% to <i>Autumnicola tepida</i> F363<sup>T</sup>. It contained menaquinone-6 (MK-6) as the sole respiratory quinone, iso-C<sub>15:0</sub> and summed feature 3 (C<sub>16:1</sub> <i>ω</i>7c and/or C<sub>16:1</sub> <i>ω</i>6c) as the major fatty acids. The main polar lipids were phosphatidylethanolamine (PE), three unidentified aminolipids (AL1-3) and four unidentified lipids (L1-4). The DNA G + C content was 39.6&#xa0;mol%. The average amino acid identity (AAI), average nucleotide identity (ANI) and digital DNA-DNA hybridization (<i>d</i>DDH) values between strain H14<sup>T</sup> and the current genome-sequenced <i>Autumnicola</i> type strains were 78.7–88.4%, 77.1–83.7%, and 17.1–45.8%, respectively. Based on the polyphasic taxonomic analysis, a novel member of the genus&#xa0;<i>Autumnicola,</i> named <i>Autumnicola aciditolerans</i> sp. nov., is proposed. The type strain is H14<sup>T</sup> (= MCCC 1K08336<sup>T</sup> = KCTC 82836<sup>&#xa0;T</sup> = CGMCC 1.61706<sup>&#xa0;T</sup>).</p>

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Autumnicola aciditolerans sp. nov., a novel member of the genus Autumnicola isolated from tidal flat in Zhoushan

  • 期刊论文

  • 发表日期：

    2026年2月21日

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  • Yang Li,
  • Dong-Yan He,
  • Jia-Wei Gao,
  • Ke Cao,
  • Yun-Fei Cao,
  • Xing-Cheng Wang,
  • Lin Xu,
  • Yan Bai,
  • Can Chen,
  • Cong Sun

摘要

A Gram-stain-negative, aerobic, and rod-shaped bacterium, designated H14T, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. Colonies of strain H14T were yellow, round, smooth and convex after cultivating on marine agar at 37 ℃ for 72 h. Cells were catalase-positive and oxidase-negative. Growth occurred at 16–40 ℃ (optimum, 37 ℃), pH 5.0–8.5 (optimum, pH 6.5) and with 0.5–10.0% (]/v) NaCl (optimum, 3.0%). Strain H14T showed highest 16S rRNA gene sequence similarity of 97.7% to Autumnicola tepida F363T. It contained menaquinone-6 (MK-6) as the sole respiratory quinone, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The main polar lipids were phosphatidylethanolamine (PE), three unidentified aminolipids (AL1-3) and four unidentified lipids (L1-4). The DNA G + C content was 39.6 mol%. The average amino acid identity (AAI), average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain H14T and the current genome-sequenced Autumnicola type strains were 78.7–88.4%, 77.1–83.7%, and 17.1–45.8%, respectively. Based on the polyphasic taxonomic analysis, a novel member of the genus Autumnicola, named Autumnicola aciditolerans sp. nov., is proposed. The type strain is H14T (= MCCC 1K08336T = KCTC 82836 T = CGMCC 1.61706 T).