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<p><i>Shewanella</i> is a diverse genus of more than 100 species known for its metabolic versatility and broad environmental distribution. Strain YIC-542<sup>T</sup>, isolated from the mussel (<i>Mytilus edulis</i>), represents a novel species, and is formally described as <i>Shewanella mytili</i> sp. nov. Strain YIC-542<sup>T</sup>, a gram-negative isolate, was cultured from the intestinal microbiota of <i>M. edulis</i>. Strain YIC-542<sup>T</sup> possesses a unipolar flagellum and comprises facultatively anaerobic, non-sporulating cells that exhibit straight or slightly curved rod shapes. It grows at temperatures ranging from 10 to 45&#xa0;°C, within a pH range of 5.0–8.0, and at NaCl concentrations of 1–7% (w/v). The 16S rRNA gene of strain YIC-542<sup>T</sup> shares the greatest nucleotide identity (98.1%) with that of <i>Shewanella yunxiaonensis</i> FJAT-54481<sup>T</sup>, and shows 98.0% identity with that of <i>Shewanella dokdonensis</i> UDC329<sup>T</sup>. The digital DNA–DNA hybridization and average nucleotide identity values between strain YIC-542<sup>T</sup> and related species were markedly lower than the established thresholds for defining a novel species. Phylogenetic analyses revealed the evolutionary relationships of strain YIC-542<sup>T</sup>, indicating that it clusters with other <i>Shewanella</i> species and is distinguishable from the type species. Strain YIC-542<sup>T</sup> exhibited a characteristic fatty acid profile, with iso-C<sub>15:0</sub> (37.3%) as the predominant component, followed by summed feature 3 (10.4%) and C<sub>17:1</sub> <i>ω</i>8<i>c</i> (8.8%). Respiratory quinones were exclusively represented by Menaquinone-7. The polar lipid profile included phosphatidylglycerol, amino lipid, aminophospholipid, phosphatidylethanolamine, and two unidentified lipids. These chemotaxonomic features, along with the genomic evidence, support the classification of this isolate as <i>Shewanella mytili</i> sp. nov. (type strain YIC-542<sup>T</sup> = KCTC 8335<sup>T</sup> = MCCC 1K08934<sup>T</sup>).</p>

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Shewanella mytili sp. nov., a novel bacterial species isolated from the intestinal tract of Mytilus edulis in the South China Sea

  • 期刊论文

  • 发表日期：

    2025年8月17日

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  • Suyun Fang,
  • Jun Min,
  • Haikun Zhang,
  • Yan Li,
  • Xingyue Lin,
  • Xiaoke Hu

摘要

Shewanella is a diverse genus of more than 100 species known for its metabolic versatility and broad environmental distribution. Strain YIC-542T, isolated from the mussel (Mytilus edulis), represents a novel species, and is formally described as Shewanella mytili sp. nov. Strain YIC-542T, a gram-negative isolate, was cultured from the intestinal microbiota of M. edulis. Strain YIC-542T possesses a unipolar flagellum and comprises facultatively anaerobic, non-sporulating cells that exhibit straight or slightly curved rod shapes. It grows at temperatures ranging from 10 to 45 °C, within a pH range of 5.0–8.0, and at NaCl concentrations of 1–7% (w/v). The 16S rRNA gene of strain YIC-542T shares the greatest nucleotide identity (98.1%) with that of Shewanella yunxiaonensis FJAT-54481T, and shows 98.0% identity with that of Shewanella dokdonensis UDC329T. The digital DNA–DNA hybridization and average nucleotide identity values between strain YIC-542T and related species were markedly lower than the established thresholds for defining a novel species. Phylogenetic analyses revealed the evolutionary relationships of strain YIC-542T, indicating that it clusters with other Shewanella species and is distinguishable from the type species. Strain YIC-542T exhibited a characteristic fatty acid profile, with iso-C15:0 (37.3%) as the predominant component, followed by summed feature 3 (10.4%) and C17:1 ω8c (8.8%). Respiratory quinones were exclusively represented by Menaquinone-7. The polar lipid profile included phosphatidylglycerol, amino lipid, aminophospholipid, phosphatidylethanolamine, and two unidentified lipids. These chemotaxonomic features, along with the genomic evidence, support the classification of this isolate as Shewanella mytili sp. nov. (type strain YIC-542T = KCTC 8335T = MCCC 1K08934T).