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<p>A Gram positive, aerobic, cream-white, motile, and rod-shaped bacterium, designated as P8<sup>T</sup>, was isolated from the soil collected from a farmland in JiangXi Province, China. Strain P8<sup>T</sup> efficiently transformed herbicide mesotrione to 2-amino-4-methylsulfonylbenzoic acid (AMBA). Strain P8<sup>T</sup> showed the highest 16S rRNA gene sequence similarity with <i>Paenibacillus terreus</i> D33<sup>T</sup> (97.4%). Phylogenetic trees based on 16S rRNA gene and genome sequences indicated that strain P8<sup>T</sup> formed a distinct clade within the genus <i>Paenibacillus</i>. The major cellular fatty acids were anteiso-C<sub>15:0</sub>, C<sub>16:0</sub>, anteiso-C<sub>17:0</sub> and iso-C<sub>16:0</sub>, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2). The DNA G + C content was 49.9%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between P8<sup>T</sup> and closely related type strains in genus <i>Paenibacillus</i> were 70.4–93.0% and 13.0–48.3%, respectively. Based on the phenotypic, phylogenetic and genomic results, strain P8<sup>T</sup> represents a novel species of the genus <i>Paenibacillus</i>, for which the name <i>Paenibacillus jiagnxiensis</i> sp. nov. is proposed, with strain P8<sup>T</sup> (= KCTC 43704<sup>&#xa0;T</sup> = MCCC 1K09190<sup>T</sup>) as the type strain. Furthermore, a nitroreductase gene (WP355320791), designated as <i>mstnr</i>, that efficiently transformed mesotrione to AMBA was identified in strain P8<sup>T</sup>. Mstnr consists of 219 amino acids, and shares low similarities (less than 32%) with reported mesotrione nitroreductases.</p>

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Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene

  • 期刊论文

  • 发表日期：

    2025年6月29日

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  • Luyao Ruan,
  • Leyun Dong,
  • Jiale Ma,
  • Ye Song,
  • Minglai Wan,
  • Jian He,
  • Jiandong Jiang

摘要

A Gram positive, aerobic, cream-white, motile, and rod-shaped bacterium, designated as P8T, was isolated from the soil collected from a farmland in JiangXi Province, China. Strain P8T efficiently transformed herbicide mesotrione to 2-amino-4-methylsulfonylbenzoic acid (AMBA). Strain P8T showed the highest 16S rRNA gene sequence similarity with Paenibacillus terreus D33T (97.4%). Phylogenetic trees based on 16S rRNA gene and genome sequences indicated that strain P8T formed a distinct clade within the genus Paenibacillus. The major cellular fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0 and iso-C16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2). The DNA G + C content was 49.9%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between P8T and closely related type strains in genus Paenibacillus were 70.4–93.0% and 13.0–48.3%, respectively. Based on the phenotypic, phylogenetic and genomic results, strain P8T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus jiagnxiensis sp. nov. is proposed, with strain P8T (= KCTC 43704 T = MCCC 1K09190T) as the type strain. Furthermore, a nitroreductase gene (WP355320791), designated as mstnr, that efficiently transformed mesotrione to AMBA was identified in strain P8T. Mstnr consists of 219 amino acids, and shares low similarities (less than 32%) with reported mesotrione nitroreductases.