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Extracellular vesicles from the mycoparasitic fungus Trichoderma harzianum

  • Gabrielle Rosa Silva,
  • Felipe de Pina Cavalcanti,
  • Reynaldo Magalhães Melo,
  • Emilio Cintra,
  • Eliana Martins Lima,
  • Pedro Ricardo Vieira Hamann,
  • Luis H. F. do Vale,
  • Cirano José Ulhoa,
  • Fausto Almeida,
  • Eliane Ferreira Noronha

摘要

Trichoderma harzianum is a filamentous fungus that can act as a mycoparasite, saprophyte, or a plant symbiotic. It is widely used as a biological control agent against phytopathogenic fungi and can also be used for plant growth promotion and biofortification. Interaction between T. harzianum and phytopathogenic fungi involves mycoparasitism, competition, and antibiosis. Extracellular vesicles (EVs) have been described as presenting a central role in mechanisms of communication and interaction among fungus and their hosts. In this study, we characterized extracellular vesicles of T. harzianum produced during growth in the presence of glucose or S. sclerotiorum mycelia. A set of vesicular proteins was identified using proteomic approach, mainly presenting predicted signal peptides.