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<p>A microfluidic system for immunohistochemistry providing improved staining uniformity and more convenient operation is designed, prototyped, and tested. The chip is comprised of two parts: a plastic (polycarbonate PC) sliding cover that forms a chamber over a glass slide with a mounted sample tissue section. Staining reagents and labeled antibodies are successively pipetted into the chamber and flow over the tissue section by gravity. Staining uniformity is improved in channel design optimization. The plastic cover includes structural features to modify the flow field and reduce the mixing of successive loadings. Flow characteristics are optimized using finite element modeling. The approach shows substantially more uniform staining, as demonstrated quantitatively by image processing of stained samples.</p>

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Flow-optimized microfluidic chip for improved immunohistochemistry analysis

  • 期刊论文

  • 发表日期：

    2025年3月6日

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  • Yijie Zhou,
  • Qiuxiang Yu,
  • Pengju Dong,
  • Zhusheng Lan,
  • Yize Zhang,
  • Tianwei Li,
  • Guijun Miao,
  • Michael G. Mauk,
  • Yuhuan Fu,
  • Ganli Nie,
  • Xin Sun,
  • Lei Wang,
  • Lulu Zhang,
  • Xianbo Qiu

摘要

A microfluidic system for immunohistochemistry providing improved staining uniformity and more convenient operation is designed, prototyped, and tested. The chip is comprised of two parts: a plastic (polycarbonate PC) sliding cover that forms a chamber over a glass slide with a mounted sample tissue section. Staining reagents and labeled antibodies are successively pipetted into the chamber and flow over the tissue section by gravity. Staining uniformity is improved in channel design optimization. The plastic cover includes structural features to modify the flow field and reduce the mixing of successive loadings. Flow characteristics are optimized using finite element modeling. The approach shows substantially more uniform staining, as demonstrated quantitatively by image processing of stained samples.