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<p>This study focuses on monitoring of SARS-CoV-2 in free-living animals in the Vale dos Sinos region, Rio Grande do Sul, Brazil, aiming to verify the presence of this emerging virus using next-generation sequencing (NGS) technique. Rectal and oral swab samples were collected from 52 white-eared opossums (<i>Didelphis albiventris</i>) and submitted to metagenomics and subsequently directed SARS-CoV-2 genome sequencing. Five positive samples were found to originate from these animals’ contact with human waste or contaminated water bodies, indicating the spillover of SARS-CoV-2 to <i>D. albiventris</i>.</p>

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SARS-CoV-2 Spillback in Opossums, Southern Brazil

  • 期刊论文

  • 发表日期：

    2025年6月22日

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  • Julyana Sthéfanie Simões Matos,
  • Meriane Demoliner,
  • Juliana Schons Gularte,
  • Micheli Filippi,
  • Vyctoria Malayhka de Abreu Góes Pereira,
  • Marcelo Pereira de Barros,
  • Fernando Rosado Spilki

摘要

This study focuses on monitoring of SARS-CoV-2 in free-living animals in the Vale dos Sinos region, Rio Grande do Sul, Brazil, aiming to verify the presence of this emerging virus using next-generation sequencing (NGS) technique. Rectal and oral swab samples were collected from 52 white-eared opossums (Didelphis albiventris) and submitted to metagenomics and subsequently directed SARS-CoV-2 genome sequencing. Five positive samples were found to originate from these animals’ contact with human waste or contaminated water bodies, indicating the spillover of SARS-CoV-2 to D. albiventris.