<p>Significant financial losses caused by human-wildlife conflict (HWC) hinder long-term conservation efforts in developing countries. HWC is a widespread and persistent challenge to conservation that adversely affects both humans and wildlife. In the current study, we assessed the nature and magnitude of HWC in Lakki Marwat, Pakistan, by interviewing 185 randomly selected participants through semi-structured questionnaires. Six animal species were identified by the locals as responsible for HWC due to livestock depredation and crop raiding: grey wolf (<i>Canis lupus</i>), golden jackal (<i>Canis aureus</i>), red fox (<i>Vulpes vulpes</i>), striped hyena (<i>Hyaena hyaena</i>), jungle cat (<i>Felis chaus</i>), and wild boar (<i>Sus scrofa</i>). Respondents reported 639 livestock losses to carnivores in the past two years (2021–2022), translating into an annual economic loss of $9,598. The grey wolf and golden jackal were responsible for 83% of the total financial loss due to livestock depredation. Location, prey type, prey age, and herding practices significantly impacted livestock depredation. Wild boar, golden jackal, and red fox caused an annual monetary loss of $4,107 through crop damage. According to local perceptions, wild boar was the most dangerous species in the study area, followed by the golden jackal and jungle cat. The majority (90%) of the respondents possessed a negative attitude towards wild boar, golden jackal, and jungle cat. Maintaining smaller herds, education, public awareness, financial incentives, and coordination between the local community and wildlife officials may reduce HWC in the study area.</p><p> <?noindent??>Clinical trial number: Not applicable.</p>