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<p>The sweet potato whitefly, <i>Bemisia tabaci</i> (Asia-I), is a&#xa0;major polyphagous pest and an efficient vector of plant viruses, causing significant crop losses. Due to widespread insecticide resistance, RNA interference (RNAi) has emerged as a&#xa0;promising alternative strategy for pest management. In the present study, five physiological genes, such as <i>α‑tubulin, ecdysone receptor (EcR), Sucrase, trehalase (TRE-1), </i>and <i>trehalose transporter (TRET-1)</i>, were targeted using orally delivered double-stranded RNA (dsRNA). Bioassays were conducted using dsRNA concentrations ranging from 0.25 to 2.0 µg/mL. A&#xa0;dose- and time-dependent increase in mortality was observed, with maximum mortality of 85.63% recorded for dsEcR at 72 h post feeding, while an optimal response was observed at 1.0 µg&#xa0;mL<sup>−1</sup> concentration. Quantitative RT-PCR analysis revealed significant gene knockdown ranging from 34.80% to 78.30% (<i>P</i> &lt; 0.05) across target genes. Among the tested targets, <i>EcR,</i> followed by <i>TRET‑1,</i> exhibited relatively higher silencing efficiency and mortality effects. These findings provide laboratory-based validation of potential RNAi targets in <i>B.&#xa0;tabaci</i> (Asia-I) and contribute to the identification of candidate genes for future RNAi-based pest management strategies.</p>

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Comparative RNAi-Mediated Silencing of Physiological Genes for the Management of Bemisia tabaci Asia-I (Gennadius) (Hemiptera: Aleyrodidae)

  • 期刊论文

  • 发表日期：

    2026年5月22日

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  • Prasad Babu Karakatti,
  • Ramasamy Asokan,
  • Karuppannasamy Ashok

摘要

The sweet potato whitefly, Bemisia tabaci (Asia-I), is a major polyphagous pest and an efficient vector of plant viruses, causing significant crop losses. Due to widespread insecticide resistance, RNA interference (RNAi) has emerged as a promising alternative strategy for pest management. In the present study, five physiological genes, such as α‑tubulin, ecdysone receptor (EcR), Sucrase, trehalase (TRE-1), and trehalose transporter (TRET-1), were targeted using orally delivered double-stranded RNA (dsRNA). Bioassays were conducted using dsRNA concentrations ranging from 0.25 to 2.0 µg/mL. A dose- and time-dependent increase in mortality was observed, with maximum mortality of 85.63% recorded for dsEcR at 72 h post feeding, while an optimal response was observed at 1.0 µg mL−1 concentration. Quantitative RT-PCR analysis revealed significant gene knockdown ranging from 34.80% to 78.30% (P < 0.05) across target genes. Among the tested targets, EcR, followed by TRET‑1, exhibited relatively higher silencing efficiency and mortality effects. These findings provide laboratory-based validation of potential RNAi targets in B. tabaci (Asia-I) and contribute to the identification of candidate genes for future RNAi-based pest management strategies.