Scholarlink

<p>Saline-alkali soil is extremely harmful for plant growth. <i>Ulmus pumila cv.‘jinye’ i</i>s a&#xa0;salt-alkali tolerant plant species with potential for the remediation of saline-alkali land. This study utilized transcriptomics and metabolomics to analyze the response mechanism of <i>Ulmus pumila cv.‘jinye’</i> under saline-alkali stress. Under stress, the leaves of <i>Ulmus pumila cv.‘jinye’ </i>exhibited a&#xa0;visible shift toward brighter yellow coloration. Concurrently, the number of differentially expressed genes (DEGs) increased significantly with higher salt-alkali concentrations. These DEGs were significantly enriched in fructose and mannose metabolism, starch and sucrose metabolism, carotenoid biosynthesis, flavonoid biosynthesis, plant hormone signal transduction, and photosynthesis. The expression levels of DEGs, including <i>alpha-trehalose-phosphate synthase, sucrose-phosphate synthase, GPDH</i>, and <i>PK</i> were significantly upregulated in response to increasing stress. However, the expression of beta-amylase and alpha-amylase genes were significantly downregulated. Notably, genes encoding <i>PSY, abscisic acid&#xa0;8′-hydroxylase&#xa0;3</i>, and <i>LECY</i> were rapidly upregulated under salt-alkali stress. Conversely, the expression of <i>beta-carotene hydroxylase&#xa0;2, chalcone-flavonone isomerase&#xa0;3, chalcone synthase, flavanone-3-hydroxylase</i>, and <i>LUTEIN DEFICIENT&#xa0;5</i> were suppressed under the high concentration stress. These candidate genes were verified by RT-PCR. Metabolomic analysis identified several key stress-responsive metabolites, including L‑cysteine, N‑acetyl-L-tryptophan, 1‑monolinolein, dihydroxyacetone phosphate, and turanose. The results of this study provide novel insights for the high-quality breeding and rational application (improvement of saline-alkali land) of <i>Ulmus pumila cv.‘jinye’</i> .</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular Dissection of Response Mechanism and Leaf Color Variation in Ulmus Pumila Cv.‘Jinye’ Under Saline-Alkali Stress

  • 期刊论文

  • 发表日期：

    2026年2月11日

查看全文
  • Shufang Yan,
  • Yichao Liu,
  • Shuxiang Feng,
  • Gongdan Zhou,
  • Yuling Li,
  • Yinran Huang

摘要

Saline-alkali soil is extremely harmful for plant growth. Ulmus pumila cv.‘jinye’ is a salt-alkali tolerant plant species with potential for the remediation of saline-alkali land. This study utilized transcriptomics and metabolomics to analyze the response mechanism of Ulmus pumila cv.‘jinye’ under saline-alkali stress. Under stress, the leaves of Ulmus pumila cv.‘jinye’ exhibited a visible shift toward brighter yellow coloration. Concurrently, the number of differentially expressed genes (DEGs) increased significantly with higher salt-alkali concentrations. These DEGs were significantly enriched in fructose and mannose metabolism, starch and sucrose metabolism, carotenoid biosynthesis, flavonoid biosynthesis, plant hormone signal transduction, and photosynthesis. The expression levels of DEGs, including alpha-trehalose-phosphate synthase, sucrose-phosphate synthase, GPDH, and PK were significantly upregulated in response to increasing stress. However, the expression of beta-amylase and alpha-amylase genes were significantly downregulated. Notably, genes encoding PSY, abscisic acid 8′-hydroxylase 3, and LECY were rapidly upregulated under salt-alkali stress. Conversely, the expression of beta-carotene hydroxylase 2, chalcone-flavonone isomerase 3, chalcone synthase, flavanone-3-hydroxylase, and LUTEIN DEFICIENT 5 were suppressed under the high concentration stress. These candidate genes were verified by RT-PCR. Metabolomic analysis identified several key stress-responsive metabolites, including L‑cysteine, N‑acetyl-L-tryptophan, 1‑monolinolein, dihydroxyacetone phosphate, and turanose. The results of this study provide novel insights for the high-quality breeding and rational application (improvement of saline-alkali land) of Ulmus pumila cv.‘jinye’ .