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<p>Myosin‑1 and polyketide synthase (PKS) proteins are the two major proteins profoundly known to facilitate fungal infection in host tissues and disease development. In our novel investigation, we co-cultured <i>C.&#xa0;falcatum</i> with fine-cut sugarcane stalks and examined the peptide abundance of myosin‑1 and PKS proteins in <i>C.&#xa0;falcatum</i> during red rot disease of sugarcane using nanoLCMS-MS technique. The results indicated the presence of eleven myosin‑1 and twenty eight polyketide synthase proteins in <i>C.&#xa0;falcatum</i>. Further, we found that two myosin‑1 (A1C4A5 and Q7SDM3) and five PKS (F9XMW3, K2QVI8, A0A2G5IC96, A7UMW1, and I1RF58) proteins were present only in the <i>C.&#xa0;falcatum</i> samples cultured with sugarcane stalks. The peptide abundance of remaining myosin‑1 and polyketide synthase proteins was significantly induced in <i>C.&#xa0;falcatum</i> samples cultured with sugarcane stalks, compared to control. The results of qRT-PCR-based real-time expression analysis of myosin‑1 (A1C4A5 and Q7SDM3), and polyketide synthase (F9XMW3 and I1RF58) genes also substantiated the findings of proteomics. String network analysis of myosin‑1 (Q7SDM3) and PKS‑1 (F9XMW3) proteins indicated a&#xa0;strong association with other proteins implicated in fungal pathogenesis. The present study suggests that myosin‑1 and polyketide synthase proteins are the two crucial proteins complicated in the process of fungal pathogenesis.</p>

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Colletotrichum falcatum Proteome Unraveled the Promising Functions of Myosin-1 and Polyketide Synthase Proteins in Instigating the Pathogen Virulence

  • 期刊论文

  • 发表日期：

    2025年4月17日

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  • Sejal Batra,
  • Ranjit Singh Gujjar,
  • Rajeev Kumar,
  • Sanjay Kumar Goswami,
  • Rahul Kumar Tiwari,
  • Appunu Chinnaswamy,
  • Ramaswamy Manimekalai,
  • Valarmathi Ramanathan,
  • Sanjeev Kumar

摘要

Myosin‑1 and polyketide synthase (PKS) proteins are the two major proteins profoundly known to facilitate fungal infection in host tissues and disease development. In our novel investigation, we co-cultured C. falcatum with fine-cut sugarcane stalks and examined the peptide abundance of myosin‑1 and PKS proteins in C. falcatum during red rot disease of sugarcane using nanoLCMS-MS technique. The results indicated the presence of eleven myosin‑1 and twenty eight polyketide synthase proteins in C. falcatum. Further, we found that two myosin‑1 (A1C4A5 and Q7SDM3) and five PKS (F9XMW3, K2QVI8, A0A2G5IC96, A7UMW1, and I1RF58) proteins were present only in the C. falcatum samples cultured with sugarcane stalks. The peptide abundance of remaining myosin‑1 and polyketide synthase proteins was significantly induced in C. falcatum samples cultured with sugarcane stalks, compared to control. The results of qRT-PCR-based real-time expression analysis of myosin‑1 (A1C4A5 and Q7SDM3), and polyketide synthase (F9XMW3 and I1RF58) genes also substantiated the findings of proteomics. String network analysis of myosin‑1 (Q7SDM3) and PKS‑1 (F9XMW3) proteins indicated a strong association with other proteins implicated in fungal pathogenesis. The present study suggests that myosin‑1 and polyketide synthase proteins are the two crucial proteins complicated in the process of fungal pathogenesis.