Scholarlink

<p><i>Elaeagnus angustifolia</i> is a key species for reforestation in arid and saline–alkaline lands and has significant ecological, medicinal and economic value. However, as a perennial woody plant, its long breeding cycle presents major challenges for the genetic improvement of economically important traits. In this study, we developed SSR markers from transcriptomic data and supplemented them with literature-reported primers, ultimately selecting 16 highly polymorphic primer pairs. Using these markers, we conducted comprehensive genetic diversity and population structure analyses on 115 <i>E. angustifolia</i> germplasm accessions collected from three core distribution regions in China. The mean values of Nei’s gene diversity (H) and Shannon diversity index (I) were 0.231 and 0.364, respectively, indicating that the <i>E. angustifolia</i> germplasm resources had a medium level of genetic diversity. Structural analysis, Neighbour-joining (NJ) cluster analysis, and principal coordinates analysis (PCoA) led to the classification of the 115 <i>E. angustifolia</i> germplasm resources into two groups. One group is composed mainly of germplasm resources from Bayingolin Mongolian Autonomous Prefecture, Xinjiang, and the other is composed mainly of germplasm resources from Changji Hui Autonomous Prefecture, Xinjiang, and Jiuquan, Gansu. AMOVA revealed that the main variation existed within groups, accounting for 80% of the total variation, while only 20% was observed between groups. These results provide support for <i>E. angustifolia</i> breeding as well as the sustainable management, protection, and utilization of its germplasm resources.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assessment of the genetic diversity and population structure of Elaeagnus angustifolia germplasm via SSR markers

  • 期刊论文

  • 发表日期：

    2026年5月13日

查看全文
  • Baocang Liu,
  • Jianhao Chen,
  • Xiangjie Wang,
  • Hongmei Luo,
  • Zhihong Liu,
  • Qing Xie

摘要

Elaeagnus angustifolia is a key species for reforestation in arid and saline–alkaline lands and has significant ecological, medicinal and economic value. However, as a perennial woody plant, its long breeding cycle presents major challenges for the genetic improvement of economically important traits. In this study, we developed SSR markers from transcriptomic data and supplemented them with literature-reported primers, ultimately selecting 16 highly polymorphic primer pairs. Using these markers, we conducted comprehensive genetic diversity and population structure analyses on 115 E. angustifolia germplasm accessions collected from three core distribution regions in China. The mean values of Nei’s gene diversity (H) and Shannon diversity index (I) were 0.231 and 0.364, respectively, indicating that the E. angustifolia germplasm resources had a medium level of genetic diversity. Structural analysis, Neighbour-joining (NJ) cluster analysis, and principal coordinates analysis (PCoA) led to the classification of the 115 E. angustifolia germplasm resources into two groups. One group is composed mainly of germplasm resources from Bayingolin Mongolian Autonomous Prefecture, Xinjiang, and the other is composed mainly of germplasm resources from Changji Hui Autonomous Prefecture, Xinjiang, and Jiuquan, Gansu. AMOVA revealed that the main variation existed within groups, accounting for 80% of the total variation, while only 20% was observed between groups. These results provide support for E. angustifolia breeding as well as the sustainable management, protection, and utilization of its germplasm resources.