Scholarlink

<p>Guava (<i>Psidium guajava</i>&#xa0;L.) is an economically important tropical fruit crop in India; however, its production and postharvest quality are severely affected by anthracnose, a&#xa0;destructive disease primarily attributed to <i>Colletotrichum</i> spp. The present study aimed to comprehensively characterize the pathogen associated with guava anthracnose in Punjab, using morphological, pathogenic, and multilocus molecular approaches. A&#xa0;total of 30&#xa0;isolates were recovered from symptomatic fruits collected across five districts of Punjab. Cultural characteristics varied among isolates, with colonies ranging from creamy white to light grey or orange, exhibiting smooth to raised growth patterns and profuse sporulation. The conidia were hyaline, cylindrical with rounded ends, and measured 10.25–15.58 × 2.76–4.73 µm. Pathogenicity assays confirmed that all isolates induced typical anthracnose symptoms, with isolates C‑2 and C‑15 being the most aggressive with maximum lesion areas of up to 41.56 mm<sup>2</sup> and a&#xa0;percent disease index of up to 72.08. Molecular characterization using internal transcribed spacer (ITS), actin (<i>ACT</i>), and β‑tubulin (<i>TUB2</i>) gene regions revealed that all isolates belonged to the <i>Colletotrichum gloeosporioides</i> species complex. Multilocus phylogenetic analysis of representative isolates, i.e., C‑2 and C‑15, confirmed their identity as <i>C.&#xa0;siamense</i>. The findings enhance understanding of the etiology of guava anthracnose in Punjab and provide a&#xa0;foundation for improved diagnostic precision and sustainable disease management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comprehensive Morphological, Pathogenic and Multilocus Molecular Characterization of Colletotrichum siamense Associated with Guava Anthracnose in Punjab, India

  • 期刊论文

  • 发表日期：

    2026年5月22日

查看全文
  • Rabia,
  • Anita Arora,
  • Amrinder Kaur,
  • Harpreet Singh,
  • Priyanka Negi,
  • Rajbir Singh Boora

摘要

Guava (Psidium guajava L.) is an economically important tropical fruit crop in India; however, its production and postharvest quality are severely affected by anthracnose, a destructive disease primarily attributed to Colletotrichum spp. The present study aimed to comprehensively characterize the pathogen associated with guava anthracnose in Punjab, using morphological, pathogenic, and multilocus molecular approaches. A total of 30 isolates were recovered from symptomatic fruits collected across five districts of Punjab. Cultural characteristics varied among isolates, with colonies ranging from creamy white to light grey or orange, exhibiting smooth to raised growth patterns and profuse sporulation. The conidia were hyaline, cylindrical with rounded ends, and measured 10.25–15.58 × 2.76–4.73 µm. Pathogenicity assays confirmed that all isolates induced typical anthracnose symptoms, with isolates C‑2 and C‑15 being the most aggressive with maximum lesion areas of up to 41.56 mm2 and a percent disease index of up to 72.08. Molecular characterization using internal transcribed spacer (ITS), actin (ACT), and β‑tubulin (TUB2) gene regions revealed that all isolates belonged to the Colletotrichum gloeosporioides species complex. Multilocus phylogenetic analysis of representative isolates, i.e., C‑2 and C‑15, confirmed their identity as C. siamense. The findings enhance understanding of the etiology of guava anthracnose in Punjab and provide a foundation for improved diagnostic precision and sustainable disease management.