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<p><i>Monilinia</i> species, which cause brown rot in pome and stone fruits around the world, lead to significant yield losses during both pre- and postharvest periods. The present study was conducted to determine the prevalence and pathogenic characteristics of <i>Monilinia laxa</i>, which causes brown rot in sweet cherry orchards in Uşak Province of Türkiye, and to determine its morphological and molecular characteristics. In this research, survey studies were conducted in 2018 and 2019 across a&#xa0;total area of 784 decares in 66&#xa0;orchards located in Uşak province and its districts (Merkez, Banaz, Eşme and Sivaslı), where most sweet cherry cultivation is carried out in April–June. <i>Monilinia </i>isolates obtained from surveyed orchards were isolated from brown rotted sweet cherry fruits. The incidence of <i>M.&#xa0;laxa</i> was highest in Sivaslı district (24.9%) in Uşak province in the first year, while it was detected in Merkez (68.8%), Sivaslı (47.6%), and Banaz (34.8%) districts in the second year, respectively. The obtained <i>Monilinia</i> isolates were identified based on their microscopic and macroscopic properties. Pathogenicity tests were performed for 52&#xa0;isolates under controlled climate chamber conditions following Koch’s postulates. In this study, morphological characterization was verified by molecular characterization using the internal transcribed spacer (ITS) region of 18S–28S nuclear ribosomal DNA, the translation elongation factor&#xa0;1-alpha (<i>tef-1α</i>) and β‑tubulin<i> (tub2</i>) genes for <i>M.&#xa0;laxa</i> and phylogenetic analysis. The multilocus matrix representation with parsimony (MRP)-based supertree was visualized using the ggtree R&#xa0;package. Integrating three gene regions into a&#xa0;single consensus topology improved the resolution and reliability of <i>Monilinia</i> species discrimination. In conclusion, considering the prevalence of fruit infections in cherry orchards is crucial for planning chemical control measures against brown rot in line with integrated pest management principles and ensuring sustainable disease control.</p>

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Characterization of Monilinia laxa Causing Brown Rot on Sweet Cherry in Uşak Province of Türkiye

  • 期刊论文

  • 发表日期：

    2026年5月28日

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  • Altınay Şimşek,
  • Havva Dinler,
  • Ayse Uysal Morca

摘要

Monilinia species, which cause brown rot in pome and stone fruits around the world, lead to significant yield losses during both pre- and postharvest periods. The present study was conducted to determine the prevalence and pathogenic characteristics of Monilinia laxa, which causes brown rot in sweet cherry orchards in Uşak Province of Türkiye, and to determine its morphological and molecular characteristics. In this research, survey studies were conducted in 2018 and 2019 across a total area of 784 decares in 66 orchards located in Uşak province and its districts (Merkez, Banaz, Eşme and Sivaslı), where most sweet cherry cultivation is carried out in April–June. Monilinia isolates obtained from surveyed orchards were isolated from brown rotted sweet cherry fruits. The incidence of M. laxa was highest in Sivaslı district (24.9%) in Uşak province in the first year, while it was detected in Merkez (68.8%), Sivaslı (47.6%), and Banaz (34.8%) districts in the second year, respectively. The obtained Monilinia isolates were identified based on their microscopic and macroscopic properties. Pathogenicity tests were performed for 52 isolates under controlled climate chamber conditions following Koch’s postulates. In this study, morphological characterization was verified by molecular characterization using the internal transcribed spacer (ITS) region of 18S–28S nuclear ribosomal DNA, the translation elongation factor 1-alpha (tef-1α) and β‑tubulin (tub2) genes for M. laxa and phylogenetic analysis. The multilocus matrix representation with parsimony (MRP)-based supertree was visualized using the ggtree R package. Integrating three gene regions into a single consensus topology improved the resolution and reliability of Monilinia species discrimination. In conclusion, considering the prevalence of fruit infections in cherry orchards is crucial for planning chemical control measures against brown rot in line with integrated pest management principles and ensuring sustainable disease control.