Scholarlink

<p>The ‘Oregon Spur’ apple cultivar, valued for its desirable traits but vulnerable to diseases, necessitates efficient propagation methods. This study aimed to establish a&#xa0;reliable micropropagation protocol and assess the genetic fidelity of <i>in vitro</i> regenerated plants. Bud explants from clonally propagated potted plants were sterilized using a&#xa0;combination of ethanol, carbendazim, and sodium hypochlorite, with the best results observed with 70% ethanol for 1 min, 0.2% carbendazim for 20 min, and 1% NaOCl for 15 min, achieving 70% uncontaminated cultures and 10% bud survival. The June-July time period of the year was found to be optimum for bud collection, which also yielded the highest bud survival (50%) and sprouting (25%). <i>In vitro</i> bud culture on Murashige and Skoog medium supplemented with 0.8 mg/l 6‑Benzyladenine (BA), 0.1 mg/l Indole-3-butyric acid (IBA), 0.5 mg/l Gibberellic acid (GA<sub>3</sub>), and 1 g/l polyvinylpyrrolidone resulted in the highest bud sprouting (42%). For shoot multiplication, the combination of 0.8 mg/l BA, 0.5 mg/l GA<sub>3</sub>, and 0.1 mg/l IBA produced the maximum shoots (5.10 shoots/explant). Rooting was optimized with 0.1 mg/l IBA, achieving 90% rooting and an average of 3.2&#xa0;roots/shoot. Acclimatization was successful in a&#xa0;mixture of cocopeat, perlite, and vermiculite (3:1:1). Genetic fidelity of the micropropagated plants was assessed using 50&#xa0;random amplified polymorphic DNA and 20&#xa0;simple sequence repeat markers. Results showed that <i>in vitro</i> propagated plants were genetically identical to the mother plant, all exhibiting monomorphic bands. This study established a&#xa0;robust micropropagation protocol for the ‘Oregon Spur’ apple cultivar, ensuring high genetic fidelity of <i>in vitro</i> produced plants. This protocol is suitable for large-scale production, providing a&#xa0;reliable method for producing disease-free, uniform planting material essential for commercial apple cultivation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In Vitro Propagation and Genetic Stability Assessment of Commercial Apple Cultivar ‘Oregon Spur’ Using Simple Sequence Repeat and Random Amplified Polymorphic DNA Markers

  • 期刊论文

  • 发表日期：

    2025年5月30日

查看全文
  • Shikha,
  • Neha Kumari,
  • Abhishek Kumar,
  • Manju Modgil

摘要

The ‘Oregon Spur’ apple cultivar, valued for its desirable traits but vulnerable to diseases, necessitates efficient propagation methods. This study aimed to establish a reliable micropropagation protocol and assess the genetic fidelity of in vitro regenerated plants. Bud explants from clonally propagated potted plants were sterilized using a combination of ethanol, carbendazim, and sodium hypochlorite, with the best results observed with 70% ethanol for 1 min, 0.2% carbendazim for 20 min, and 1% NaOCl for 15 min, achieving 70% uncontaminated cultures and 10% bud survival. The June-July time period of the year was found to be optimum for bud collection, which also yielded the highest bud survival (50%) and sprouting (25%). In vitro bud culture on Murashige and Skoog medium supplemented with 0.8 mg/l 6‑Benzyladenine (BA), 0.1 mg/l Indole-3-butyric acid (IBA), 0.5 mg/l Gibberellic acid (GA3), and 1 g/l polyvinylpyrrolidone resulted in the highest bud sprouting (42%). For shoot multiplication, the combination of 0.8 mg/l BA, 0.5 mg/l GA3, and 0.1 mg/l IBA produced the maximum shoots (5.10 shoots/explant). Rooting was optimized with 0.1 mg/l IBA, achieving 90% rooting and an average of 3.2 roots/shoot. Acclimatization was successful in a mixture of cocopeat, perlite, and vermiculite (3:1:1). Genetic fidelity of the micropropagated plants was assessed using 50 random amplified polymorphic DNA and 20 simple sequence repeat markers. Results showed that in vitro propagated plants were genetically identical to the mother plant, all exhibiting monomorphic bands. This study established a robust micropropagation protocol for the ‘Oregon Spur’ apple cultivar, ensuring high genetic fidelity of in vitro produced plants. This protocol is suitable for large-scale production, providing a reliable method for producing disease-free, uniform planting material essential for commercial apple cultivation.