Scholarlink

<p>Repeated use of synthetic insecticides for controlling insect pests leads to the development of resistance, which is detrimental to the environment and human health. Therefore, it is necessary to identify alternative botanical lead (s) for the control of sucking pests. In this study, the GC–MS-based characterization and insecticidal potential of extracts, fractions and isolated compounds of <i>Achyranthes bidentata</i> Blume seeds, roots and stems were investigated against <i>Aphis craccivora</i> Koch and <i>Planococcus lilacinus</i> Cockrell. The results showed that the GC–MS based characterization of nonpolar fractions (<i>n</i>-hexane) of seed, roots and stem was done which revealed <i>n</i>-hexadecanoic acid, (Z)-9-octadecenoic acid, hexadecanoic acid ethyl ester, (Z, Z)-9,12-octadecadienoic acid ethyl ester and (E, E)-2,4-decadienal as major bioactive components, comprising 80.3–95.6%. In contact toxicity studies, the seed ethyl acetate fraction (SEAF) was found to be more effective against <i>A. craccivora</i> (LD<sub>50</sub> = 0.73&#xa0;µg/insect) after 96&#xa0;h of treatment, followed by the seed hexane fraction (SHF), LD<sub>50</sub> = 1.13&#xa0;µg/insect. In <i>P. lilacinus</i>, SEAF demonstrated greater efficacy (LD<sub>50</sub> = 1.15&#xa0;µg/insect), followed by the root ethyl acetate fraction (REAF) (LD<sub>50</sub> = 1.57&#xa0;µg/insect). Among the compounds isolated, compound <b>3</b> (3-<i>O</i>-[<i>β</i>-D-xylopyranosyl-(1 → 2)]-[<i>α</i>-L-rhamnopyranosyl-(1 → 3)]-<i>β</i>-D-glucuronopyranosyl-28-<i>O</i>-<i>β</i>-D-glucopyranoside-3<i>β</i>-hydroxy-olean-12-en-28-oate) exhibited promising activity against <i>A. craccivora</i> and <i>P. lilacinus</i> (LD<sub>50</sub> = 0.62 and 0.72&#xa0;µg/insect, respectively), followed by compound 6 (<i>N-cis</i>-feruloyltyramine), LD<sub>50</sub> = 0.82 and 1.03&#xa0;µg/insect, respectively. Higher concentrations of extracts, fractions and isolated compounds exhibited a stronger inhibition capacity. The mechanism of insecticidal action of seed parent extract (SPE), stem parent extract (STPE) and compound 3 was mediated by inhibition of acetylcholinesterase (AChE), glutathione-<i>S</i>-transferase (GST) and mixed function oxidase (MFO) in test insects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Insecticidal activities and mechanism of action of extracts, fractions and isolated compounds from Achyranthes bidentata against Aphis craccivora and Planococcus lilacinus

  • 期刊论文

  • 发表日期：

    2025年9月22日

查看全文
  • Shivani Puri,
  • Urvashi Kashyap,
  • Upendra Sharma,
  • S. G. Eswara Reddy

摘要

Repeated use of synthetic insecticides for controlling insect pests leads to the development of resistance, which is detrimental to the environment and human health. Therefore, it is necessary to identify alternative botanical lead (s) for the control of sucking pests. In this study, the GC–MS-based characterization and insecticidal potential of extracts, fractions and isolated compounds of Achyranthes bidentata Blume seeds, roots and stems were investigated against Aphis craccivora Koch and Planococcus lilacinus Cockrell. The results showed that the GC–MS based characterization of nonpolar fractions (n-hexane) of seed, roots and stem was done which revealed n-hexadecanoic acid, (Z)-9-octadecenoic acid, hexadecanoic acid ethyl ester, (Z, Z)-9,12-octadecadienoic acid ethyl ester and (E, E)-2,4-decadienal as major bioactive components, comprising 80.3–95.6%. In contact toxicity studies, the seed ethyl acetate fraction (SEAF) was found to be more effective against A. craccivora (LD50 = 0.73 µg/insect) after 96 h of treatment, followed by the seed hexane fraction (SHF), LD50 = 1.13 µg/insect. In P. lilacinus, SEAF demonstrated greater efficacy (LD50 = 1.15 µg/insect), followed by the root ethyl acetate fraction (REAF) (LD50 = 1.57 µg/insect). Among the compounds isolated, compound 3 (3-O-[β-D-xylopyranosyl-(1 → 2)]-[α-L-rhamnopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl-28-O-β-D-glucopyranoside-3β-hydroxy-olean-12-en-28-oate) exhibited promising activity against A. craccivora and P. lilacinus (LD50 = 0.62 and 0.72 µg/insect, respectively), followed by compound 6 (N-cis-feruloyltyramine), LD50 = 0.82 and 1.03 µg/insect, respectively. Higher concentrations of extracts, fractions and isolated compounds exhibited a stronger inhibition capacity. The mechanism of insecticidal action of seed parent extract (SPE), stem parent extract (STPE) and compound 3 was mediated by inhibition of acetylcholinesterase (AChE), glutathione-S-transferase (GST) and mixed function oxidase (MFO) in test insects.