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<p>A novel online preparative comprehensive two-dimensional liquid chromatography (2D-LC) method coupling normal-phase liquid chromatography (NPLC) and reversed-phase liquid chromatography (RPLC) has been developed using an innovative interface design. Based on a previously reported vacuum evaporation-assisted adsorption (VEAA) system, this interface simplifies the overall 2D-LC configuration by enabling the second-dimensional RPLC to share a binary pump with the NP–RP transition unit. Controlled by a special elution program, the binary pump delivers solvents for both RPLC column elution and NP–RP conversion. This integration significantly reduces system complexity. The efficacy of the system was demonstrated through preparative isolation of an ethyl acetate extract of <i>Escherichia coli</i>, yielding 28 high-purity compounds, thereby confirming the reliability of the NPLC × RPLC platform.</p>

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Online Comprehensive Two-Dimensional Normal-Phase × Reversed-Phase Preparative Liquid Chromatography System with a Novel Design Interface

  • 期刊论文

  • 发表日期：

    2025年11月2日

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  • Su-Zhi Xie,
  • Mei-Juan Fang,
  • Zheng Wang,
  • Xia Yan,
  • Ying-Kun Qiu

摘要

A novel online preparative comprehensive two-dimensional liquid chromatography (2D-LC) method coupling normal-phase liquid chromatography (NPLC) and reversed-phase liquid chromatography (RPLC) has been developed using an innovative interface design. Based on a previously reported vacuum evaporation-assisted adsorption (VEAA) system, this interface simplifies the overall 2D-LC configuration by enabling the second-dimensional RPLC to share a binary pump with the NP–RP transition unit. Controlled by a special elution program, the binary pump delivers solvents for both RPLC column elution and NP–RP conversion. This integration significantly reduces system complexity. The efficacy of the system was demonstrated through preparative isolation of an ethyl acetate extract of Escherichia coli, yielding 28 high-purity compounds, thereby confirming the reliability of the NPLC × RPLC platform.