Scholarlink

<p>A straightforward and precise technique for the analysis of Rose Bengal concentrations in canine plasma was established and validated using HPLC. Following a simple protein precipitation method with methanol, separation occurred on an XBridge Phenyl column with a mobile phase of 10&#xa0;mM ammonium phosphate and acetonitrile (65:35, v/v). Rose Bengal and meloxicam, the internal standard, were confirmed by monitoring UV–visible wavelengths at 549&#xa0;nm and 370&#xa0;nm, respectively. The lower limit of quantification (LLOQ) was determined to be 25&#xa0;ng&#xa0;mL<sup>−1</sup> in a 100&#xa0;μL sample. The recovery was greater than 90% while intra-assay variability was less than 5%. Inter-assay variability was less than 7%. This method could be useful in pharmacokinetic studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and Validation of a High-Performance Liquid Chromatography Method for the Detection of Rose Bengal in Canine Plasma

  • 期刊论文

  • 发表日期：

    2025年8月21日

查看全文
  • Sherry Cox,
  • Madeline Duncan,
  • Riley Golias,
  • Julia Cutchin,
  • Nora Springer,
  • Aaron Bloom,
  • Joan Bergman

摘要

A straightforward and precise technique for the analysis of Rose Bengal concentrations in canine plasma was established and validated using HPLC. Following a simple protein precipitation method with methanol, separation occurred on an XBridge Phenyl column with a mobile phase of 10 mM ammonium phosphate and acetonitrile (65:35, v/v). Rose Bengal and meloxicam, the internal standard, were confirmed by monitoring UV–visible wavelengths at 549 nm and 370 nm, respectively. The lower limit of quantification (LLOQ) was determined to be 25 ng mL−1 in a 100 μL sample. The recovery was greater than 90% while intra-assay variability was less than 5%. Inter-assay variability was less than 7%. This method could be useful in pharmacokinetic studies.