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<p>Circulating tumor cells (CTCs) and blood plasma both work as biomarkers for the initial detection and diagnosis of metastatic cancers. Nowadays, with the development of microscale devices, for the separation of CTCs and blood plasma from normal cells, one of the most effective techniques is inertial focusing using microfluidic chips. The present research work reported a single inlet and single loop-based microfluidic chip for simultaneous separation of PC3 (prostate cancer) CTCs and blood plasma from normal blood cells using the inertial focusing approach. Two different sized PC3 CTCs and different inlet flow velocities corresponding to different Reynolds numbers are explored to accomplish optimum separation performance of the chip. Three-dimensional numerical simulations were executed with COMSOL Multiphysics 5.4 software and finite element method analysis. It has been confirmed that 100 % separation purity and cell recovery were achieved for both 17 µm and 20 µm PC3 CTCs at the Reynolds number of 67 and 65.67, respectively. The microfluidic chip also achieved a maximum plasma recovery of 54.87 % with 100 % purity. </p>

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Design and Numerical Analysis of a Single Inlet-Based Spiral Microfluidic Chip for Separation of Circulating Tumor Cells and Blood Plasma Using the Inertial Focusing Approach

  • 期刊论文

  • 发表日期：

    2025年5月22日

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  • Writtick Pakhira,
  • R. Kumar,
  • Khalid Mohd. Ibrahimi,
  • Rituraj Bhattacharjee

摘要

Circulating tumor cells (CTCs) and blood plasma both work as biomarkers for the initial detection and diagnosis of metastatic cancers. Nowadays, with the development of microscale devices, for the separation of CTCs and blood plasma from normal cells, one of the most effective techniques is inertial focusing using microfluidic chips. The present research work reported a single inlet and single loop-based microfluidic chip for simultaneous separation of PC3 (prostate cancer) CTCs and blood plasma from normal blood cells using the inertial focusing approach. Two different sized PC3 CTCs and different inlet flow velocities corresponding to different Reynolds numbers are explored to accomplish optimum separation performance of the chip. Three-dimensional numerical simulations were executed with COMSOL Multiphysics 5.4 software and finite element method analysis. It has been confirmed that 100 % separation purity and cell recovery were achieved for both 17 µm and 20 µm PC3 CTCs at the Reynolds number of 67 and 65.67, respectively. The microfluidic chip also achieved a maximum plasma recovery of 54.87 % with 100 % purity.