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Objective <p>Accurate prediction of the response to nano-photothermal therapy (NPTT) requires rapid feedback from the tumor. This study utilizes proton magnetic resonance spectroscopy (<sup>1</sup>H-MRS) to non-invasively assess metabolic changes in glioma treated with NPTT using gold-coated iron oxide nanoparticles, providing insight into early therapeutic efficacy.</p> Materials and methods <p>The study was conducted on nude mice bearing U87-MG human glioma xenografts, which were assigned to distinct treatment cohorts: Au@Fe₂O₃ core–shell nanoparticles alone, laser irradiation alone, and the combined nano-photothermal therapy. The efficacy was determined by assessing post-treatment alterations in tumor volume and corresponding changes in choline and lipid metabolite signals detected via <sup>1</sup>H-MRS.</p> Results <p>Analysis of metabolite ratios, normalized to the water peak, demonstrated a significant post-NPTT therapeutic response. A marked decrease was observed in both the choline/creatine ratio (from 0.48 ± 0.17 to 0.24 ± 0.07) and the choline/lipid ratio (from 0.47 ± 0.23 to 0.13 ± 0.06). Notably, these metabolic alterations were evident within 24&#xa0;h of the procedure. In contrast, significant reductions in tumor volume were not detectable until day 9 post-treatment.</p> Conclusion <p><sup>1</sup>H-MRS analysis of metabolite ratios serves as a sensitive, early biomarker of the biological response to NPTT. The capacity for in vivo assessment of therapeutic efficacy within 24&#xa0;h post-procedure provides a significant advantage. This rapid feedback can critically inform clinical decision-making and guide the strategic planning of subsequent cancer treatments.</p>

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Application of magnetic resonance spectroscopy (MRS) in photo-thermal therapy response of U87-MG human glioma cells with gold-coated iron oxide nanoparticles: an in vivo study

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Erfan Saatchian,
  • Hassan Tavakoli,
  • Alireza Keramati,
  • Mohammad Mahdi Modarres Mosalla,
  • Alireza Montazerabadi,
  • Hamid Fakhimi Kabir,
  • Masumeh Goodarzi

摘要

Objective

Accurate prediction of the response to nano-photothermal therapy (NPTT) requires rapid feedback from the tumor. This study utilizes proton magnetic resonance spectroscopy (1H-MRS) to non-invasively assess metabolic changes in glioma treated with NPTT using gold-coated iron oxide nanoparticles, providing insight into early therapeutic efficacy.

Materials and methods

The study was conducted on nude mice bearing U87-MG human glioma xenografts, which were assigned to distinct treatment cohorts: Au@Fe₂O₃ core–shell nanoparticles alone, laser irradiation alone, and the combined nano-photothermal therapy. The efficacy was determined by assessing post-treatment alterations in tumor volume and corresponding changes in choline and lipid metabolite signals detected via 1H-MRS.

Results

Analysis of metabolite ratios, normalized to the water peak, demonstrated a significant post-NPTT therapeutic response. A marked decrease was observed in both the choline/creatine ratio (from 0.48 ± 0.17 to 0.24 ± 0.07) and the choline/lipid ratio (from 0.47 ± 0.23 to 0.13 ± 0.06). Notably, these metabolic alterations were evident within 24 h of the procedure. In contrast, significant reductions in tumor volume were not detectable until day 9 post-treatment.

Conclusion

1H-MRS analysis of metabolite ratios serves as a sensitive, early biomarker of the biological response to NPTT. The capacity for in vivo assessment of therapeutic efficacy within 24 h post-procedure provides a significant advantage. This rapid feedback can critically inform clinical decision-making and guide the strategic planning of subsequent cancer treatments.