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<p><i>Phytophthora infestans</i> causes late blight of potato plants that can lead to decay of stored tubers, which are prone to secondary fungal or bacterial infections and subsequent decay. The bacteria responsible for this secondary decay have not been identified. In this study, we assessed whether the presence of endophytic bacteria promotes bacterial decay in tubers infected with <i>Ph. infestans</i>. We reproduced storage decay by inoculating tubers with <i>Ph. infestans</i>. Eleven bacteria isolated from these decayed tubers were then tested on tuber slices for their ability to cause decay; the isolates induced decay in tubers previously inoculated with <i>Ph. infestans</i> but not in control tubers that were not inoculated with <i>Ph. infestans</i>. To evaluate the impact of bacterial inoculation and <i>Ph. infestans</i>, we also weighed the exudate from decayed tissue. More exudate was produced by samples inoculated with both <i>Ph. infestans</i> and bacteria than those inoculated only with bacteria. Phylogenetic analysis based on 16S rRNA and <i>rpoB</i> sequences revealed that bacterial isolates were closely related to <i>Paenibacillus polymyxa</i>. Characteristics of three representative bacterial isolates were also consistent with those of <i>Pa. polymyxa</i> ATCC 842<sup>T</sup>. Real-time polymerase chain reactions confirmed that <i>Pa. polymyxa</i> proliferated in tubers infected with <i>Ph. infestans</i>, although tubers were not inoculated with the bacterium. Together, these findings indicate that the presence and proliferation of endophytic <i>Pa. polymyxa</i> contribute to storage decay in potato tubers infected by <i>Ph. infestans</i>.</p>

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Potential role of Paenibacillus polymyxa in spread of decay among stored potato tubers infected with Phytophthora infestans

  • 期刊论文

  • 发表日期：

    2026年4月22日

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  • Ryuto Yoshida,
  • Norio Kondo,
  • Seishi Akino

摘要

Phytophthora infestans causes late blight of potato plants that can lead to decay of stored tubers, which are prone to secondary fungal or bacterial infections and subsequent decay. The bacteria responsible for this secondary decay have not been identified. In this study, we assessed whether the presence of endophytic bacteria promotes bacterial decay in tubers infected with Ph. infestans. We reproduced storage decay by inoculating tubers with Ph. infestans. Eleven bacteria isolated from these decayed tubers were then tested on tuber slices for their ability to cause decay; the isolates induced decay in tubers previously inoculated with Ph. infestans but not in control tubers that were not inoculated with Ph. infestans. To evaluate the impact of bacterial inoculation and Ph. infestans, we also weighed the exudate from decayed tissue. More exudate was produced by samples inoculated with both Ph. infestans and bacteria than those inoculated only with bacteria. Phylogenetic analysis based on 16S rRNA and rpoB sequences revealed that bacterial isolates were closely related to Paenibacillus polymyxa. Characteristics of three representative bacterial isolates were also consistent with those of Pa. polymyxa ATCC 842T. Real-time polymerase chain reactions confirmed that Pa. polymyxa proliferated in tubers infected with Ph. infestans, although tubers were not inoculated with the bacterium. Together, these findings indicate that the presence and proliferation of endophytic Pa. polymyxa contribute to storage decay in potato tubers infected by Ph. infestans.