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<p>Here we studied the mechanisms underlying the antifungal activity of traversianal, a fungal diterpenoid aldehyde, against <i>Colletotrichum orbiculare</i> and tested its activity against three bacterial species. In vitro, traversianal only inhibited growth of the <i>Bacillus</i> species; it had no effect on two <i>Pseudomonas</i> species. Treatment of <i>C. orbiculare</i> with transverianal and lecithin, which consists primarily of phospholipids, attenuated the fungicidal activity of transverianal; significantly more colonies formed on potato sucrose agar after treatment with traversianal in the presence of lecithin, thus indicating competitive inhibition of fungicidal activity by lecithin. However, l-α-phosphatidylethanolamine dioleoyl, but not l-α-phosphatidylcholine dioleoyl, had a similar antagonistic effect. These findings provide evidence that the cell membrane component phosphatidylethanolamine may be specifically targeted in the fungicidal activity of traversianal, which is consistent with our transmission electron microscopy observations revealing the fragmentation of plasma membranes and disappearance of cell organelles in the conidia of <i>C. orbiculare</i> treated with traversianal. Thus, traversianal may have potential application as a novel fungicide that targets phosphatidylethanolamine.</p>

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Mechanism of the antifungal activity of the diterpenoid aldehyde traversianal against cucumber anthracnose fungus Colletotrichum orbiculare

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Masatoshi Ino,
  • Junichi Kihara,
  • Atsushi Ishihara,
  • Makoto Ueno

摘要

Here we studied the mechanisms underlying the antifungal activity of traversianal, a fungal diterpenoid aldehyde, against Colletotrichum orbiculare and tested its activity against three bacterial species. In vitro, traversianal only inhibited growth of the Bacillus species; it had no effect on two Pseudomonas species. Treatment of C. orbiculare with transverianal and lecithin, which consists primarily of phospholipids, attenuated the fungicidal activity of transverianal; significantly more colonies formed on potato sucrose agar after treatment with traversianal in the presence of lecithin, thus indicating competitive inhibition of fungicidal activity by lecithin. However, l-α-phosphatidylethanolamine dioleoyl, but not l-α-phosphatidylcholine dioleoyl, had a similar antagonistic effect. These findings provide evidence that the cell membrane component phosphatidylethanolamine may be specifically targeted in the fungicidal activity of traversianal, which is consistent with our transmission electron microscopy observations revealing the fragmentation of plasma membranes and disappearance of cell organelles in the conidia of C. orbiculare treated with traversianal. Thus, traversianal may have potential application as a novel fungicide that targets phosphatidylethanolamine.