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<p>Antimicrobial resistant (AMR) bacteria is the common threat in clinical, livestock breeding, aquacultural, and agricultural fields. To prepare for new AMR bacteria, we developed a simple and convenient method for antimicrobial susceptibility testing (AST) of plant pathogenic bacteria. The new recipe for media preparation and bacterial inoculation was proposed with understandable instructions, based on Goto and Takikawa (<CitationRef CitationID="CR7">1984</CitationRef>). Nutrient agar with 1% glucose were equivalent to the original medium. Inoculation using cotton swabs allow users to observe clear inhibition zones. The AST method for phytopathogenic bacteria was not standardized so far, but new method enables comparison and accumulation of data.</p>

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A simple and convenient method for antimicrobial susceptibility testing of plant pathogenic bacteria

  • 期刊论文

  • 发表日期：

    2025年6月18日

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  • Mizue Tsuji,
  • Takashi Fujikawa,
  • Yasuhiro Inoue,
  • Yuichi Takikawa

摘要

Antimicrobial resistant (AMR) bacteria is the common threat in clinical, livestock breeding, aquacultural, and agricultural fields. To prepare for new AMR bacteria, we developed a simple and convenient method for antimicrobial susceptibility testing (AST) of plant pathogenic bacteria. The new recipe for media preparation and bacterial inoculation was proposed with understandable instructions, based on Goto and Takikawa (1984). Nutrient agar with 1% glucose were equivalent to the original medium. Inoculation using cotton swabs allow users to observe clear inhibition zones. The AST method for phytopathogenic bacteria was not standardized so far, but new method enables comparison and accumulation of data.