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<p>In this first report of pepper co-infected with potyviruses chilli veinal mottle virus (ChiVMV), chilli ringspot virus (ChiRSV), and wild tomato mosaic virus (WTMV), a rapid multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed for simultaneous detection of the viruses using four pairs of specific primers and reference gene ubiquitin-conjugating protein (<i>UBI</i>) in a single reaction tube. With optimized primer concentrations and annealing temperatures, 100&#xa0;fg was the lowest template concentration to simultaneously detect ChiVMV, ChiRSV, and WTMV. This multiplex RT‑PCR was then used in the field to accurately diagnose the three viruses coinfecting pepper samples and is an effective tool for conventional molecular diagnosis and epidemiological studies of ChiVMV, ChiRSV, and WTMV in pepper.</p>

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A multiplex RT‑PCR detection system for pepper co-infection by three potyviruses

  • 期刊论文

  • 发表日期：

    2025年2月10日

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  • Tiyuan Xia,
  • Liling Mo,
  • Xinyang Chen,
  • Wang Wang,
  • Pengyu Liu,
  • Xingmei Tao,
  • Xuan Yi,
  • Zuqin Qiao,
  • Yongfu Zhang,
  • Xiaoqin Li

摘要

In this first report of pepper co-infected with potyviruses chilli veinal mottle virus (ChiVMV), chilli ringspot virus (ChiRSV), and wild tomato mosaic virus (WTMV), a rapid multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed for simultaneous detection of the viruses using four pairs of specific primers and reference gene ubiquitin-conjugating protein (UBI) in a single reaction tube. With optimized primer concentrations and annealing temperatures, 100 fg was the lowest template concentration to simultaneously detect ChiVMV, ChiRSV, and WTMV. This multiplex RT‑PCR was then used in the field to accurately diagnose the three viruses coinfecting pepper samples and is an effective tool for conventional molecular diagnosis and epidemiological studies of ChiVMV, ChiRSV, and WTMV in pepper.