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<p>The viability and virulence of <i>Calonectria ilicicola</i> cultured with barley grains were examined after storage at −&#xa0;20&#xa0;°C for up to 19&#xa0;years. Of 114 isolates, 93.0% remained viable, and their virulence over 19&#xa0;years was comparable to that at the start of culturing and after 3&#xa0;years of storage on barley grains. The isolates colonized various parts of the barley grains, but survival was highest in the endosperm. This method is thus highly effective for long-term, stable preservation of <i>C. ilicicola</i>.</p>

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Long-term preservation method for Calonectria ilicicola using barley grains

  • 期刊论文

  • 发表日期：

    2025年2月8日

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  • Sunao Ochi

摘要

The viability and virulence of Calonectria ilicicola cultured with barley grains were examined after storage at − 20 °C for up to 19 years. Of 114 isolates, 93.0% remained viable, and their virulence over 19 years was comparable to that at the start of culturing and after 3 years of storage on barley grains. The isolates colonized various parts of the barley grains, but survival was highest in the endosperm. This method is thus highly effective for long-term, stable preservation of C. ilicicola.