Genome sequence of strawberry pseudo mild yellow edge virus isolated in Japan

The almost-complete genome sequence of strawberry pseudo mild yellow edge virus (SPMYEV), isolated in Japan, was determined via high-throughput sequencing using purified virus sourced in 1982. An 8598 nt-long sequence with a poly-A tail was obtained and potentially encodes an RNA-dependent replicase (Rep), triple gene block (TGB) 1 to 3, coat protein (CP), and nucleic acid-binding (NB) protein, with sequence similarity to Carlavirus Rep and CP (< 50% amino acid sequence identity). Percentage identity matrix analysis of the deduced amino acid sequences of Rep and CP revealed that SPMYEV is a distinct member of the genus Carlavirus in the Betaflexiviridae family.