<p>Soil carbon sequestration is critical for climate mitigation, yet the effect of dissolved inorganic carbon on the stability of mineral-associated organic matter is unclear. We hypothesized that dissolved inorganic carbon promotes the desorption of mineral-associated organic matter. We performed batch desorption experiments with soil mineral particles and soil incubation experiments with ¹³C-labeled glucose at dissolved inorganic carbon concentrations of 0–0.06&#xa0;mol/L. Organic matter was analyzed by Fourier-transform ion cyclotron resonance mass spectrometry and X-ray photoelectron spectroscopy. Results showed that 0.06&#xa0;mol/L dissolved inorganic carbon released 10.6% of mineral-associated organic carbon, a release value similar to the use of phosphate, of 12.8%. Dissolved inorganic carbon preferentially mobilized lignin- and condensed aromatic-like molecules, while phosphate released diverse biomolecules. High levels of dissolved inorganic carbon inhibited ¹³C-glucose incorporation into mineral-associated organic matter, and induced a decline of total mineral-associated organic carbon via a priming effect.</p>