Scholarlink

<p>Plant hormones play critical roles in many aspects of plant life cycles including development, growth, reproduction and responses to environmental stimuli. These processes are often associated with changes in endogenous plant hormone levels and locations. Therefore, to understand the modes of action of plant hormones, it is important to accurately quantify these chemical compounds in a high-definition tissue map. In this study, we developed a system to quantify indole-3-acetic acid (IAA), the major endogenous auxin, from small tissue samples using laser microdissection (LMD) coupled with nano-flow liquid chromatography (nano-LC)-mass spectrometry (MS), which improved detection limits, allowing quantification of IAA from a single 10&#xa0;μm cryosection of maize coleoptile. Our results reveal that IAA is actively synthesized in the apical 400&#xa0;μm region of the coleoptiles and is preferentially accumulated in vascular tissues. This technique can provide a precise view of the spatiotemporal distribution of plant hormones and their significance in regulating physiological responses at tissue or cellular levels.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Nano-flow liquid chromatography-mass spectrometry for highly sensitive quantification of indole-3-acetic acid from small tissue sections prepared by laser micro dissection

  • 期刊论文

  • 发表日期：

    2026年7月20日

查看全文
  • Hiromi Suzuki,
  • Yumiko Takebayashi,
  • Shunsuke Watanabe,
  • Noriko Takeda-Kamiya,
  • Kiminori Toyooka,
  • Masami Yokota Hirai,
  • Mitsunori Seo

摘要

Plant hormones play critical roles in many aspects of plant life cycles including development, growth, reproduction and responses to environmental stimuli. These processes are often associated with changes in endogenous plant hormone levels and locations. Therefore, to understand the modes of action of plant hormones, it is important to accurately quantify these chemical compounds in a high-definition tissue map. In this study, we developed a system to quantify indole-3-acetic acid (IAA), the major endogenous auxin, from small tissue samples using laser microdissection (LMD) coupled with nano-flow liquid chromatography (nano-LC)-mass spectrometry (MS), which improved detection limits, allowing quantification of IAA from a single 10 μm cryosection of maize coleoptile. Our results reveal that IAA is actively synthesized in the apical 400 μm region of the coleoptiles and is preferentially accumulated in vascular tissues. This technique can provide a precise view of the spatiotemporal distribution of plant hormones and their significance in regulating physiological responses at tissue or cellular levels.