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<p>The collection of root exudates, particularly those from plants symbiotically associated with arbuscular mycorrhizal fungi (AMF), is notably challenging. A semi-hydroponic cultivation system (SHCS) was designed to collect rhizosphere exudates from maize in symbiosis with AMF. This system utilizes perlite as a solid support to simulate soil barriers, combined with drip irrigation to facilitate symbiosis and the collection of maize root exudates. SHCS consists of a culture bottle, a solution bottle providing nutrients, a peristaltic pump for powering the system, silicone tubes connecting all components, a flat-jaw pinchcock for operation, and a device shelf for placing all items. Then it was used to collect root exudates from maize-wild type B73 and AMF-inoculated B73, followed by metabolomics analysis using LC-MS/MS. Through comparative analysis, we identified significant differences in metabolite levels between B73 and RiB73. Briefly, a total of 54 metabolites exhibited AMF-related characteristics, and these metabolites were enriched in 15 metabolic pathways. Key metabolites include lumichrome, riboflavin, indolelactic acid, abscisic acid, gibberellin a116, and l-histidinol phosphate. Among them, l-histidinol phosphate significantly decreased after AMF inoculation, while the other metabolites showed a notable increase in content.</p>

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A semi-hydroponic cultivation system designed for collecting root exudates from maize in symbiosis with arbuscular mycorrhiza fungi

  • 期刊论文

  • 发表日期：

    2026年4月19日

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  • Xinhao Luo,
  • Xiaowan Geng,
  • Jing Zhou,
  • Jin Chen,
  • Beijiu Cheng,
  • Xiaoyu Li

摘要

The collection of root exudates, particularly those from plants symbiotically associated with arbuscular mycorrhizal fungi (AMF), is notably challenging. A semi-hydroponic cultivation system (SHCS) was designed to collect rhizosphere exudates from maize in symbiosis with AMF. This system utilizes perlite as a solid support to simulate soil barriers, combined with drip irrigation to facilitate symbiosis and the collection of maize root exudates. SHCS consists of a culture bottle, a solution bottle providing nutrients, a peristaltic pump for powering the system, silicone tubes connecting all components, a flat-jaw pinchcock for operation, and a device shelf for placing all items. Then it was used to collect root exudates from maize-wild type B73 and AMF-inoculated B73, followed by metabolomics analysis using LC-MS/MS. Through comparative analysis, we identified significant differences in metabolite levels between B73 and RiB73. Briefly, a total of 54 metabolites exhibited AMF-related characteristics, and these metabolites were enriched in 15 metabolic pathways. Key metabolites include lumichrome, riboflavin, indolelactic acid, abscisic acid, gibberellin a116, and l-histidinol phosphate. Among them, l-histidinol phosphate significantly decreased after AMF inoculation, while the other metabolites showed a notable increase in content.