Scholarlink

<p>Clinical outcomes after transarterial chemoembolization (TACE) for intermediate–to–advanced hepatocellular carcinoma (HCC) are highly variable, and reliable predictive biomarkers are lacking. Although gut microbiota dysbiosis has been implicated in HCC pathogenesis and immunotherapy response, its role in TACE outcomes remains unclear. This study aimed to delineate gut microbiome remodeling across HCC progression and TACE treatment and to evaluate its association with clinical outcomes. Fecal samples were collected from healthy controls (<i>n</i> = 10), newly diagnosed untreated HCC patients (<i>n</i> = 30), post-TACE HCC patients (<i>n</i> = 29), and a longitudinal cohort of TACE-treated patients (<i>n</i> = 56). Gut microbiota profiles were analyzed using 16S rDNA sequencing. Differential taxa, microbial diversity, functional pathways, and survival associations were assessed using bioinformatics and Kaplan–Meier analyses. Gut microbiome composition differed markedly across healthy controls, untreated HCC, and post-TACE patients. Bacteroides abundance progressively decreased, whereas Prevotella_9 increased across disease progression and treatment. Among TACE-treated patients, progressive disease was characterized by higher Prevotella_9 and lower Bacteroides and Ruminococcus abundance, accompanied by significantly reduced microbial diversity. Kaplan–Meier analysis showed that low Prevotella_9 (<i>P</i> = 0.001) and high Bacteroides (<i>P</i> = 0.001) and <i>Faecalibacterium</i> (<i>P</i> = 0.04) were associated with prolonged progression-free survival, while higher <i>Phascolarctobacterium</i> and <i>Veillonella</i> abundance correlated with shorter overall survival. Functional prediction revealed alterations in pathways related to lipid metabolism, carbohydrate metabolism, and immune regulation. Gut microbiome remodeling is closely associated with HCC progression and TACE outcomes. Specific microbial taxa, particularly Bacteroides and Prevotella_9, were associated with therapeutic response and prognosis; however, these findings are exploratory and warrant We hypothesized that specific microbial taxa and metabolic pathways could serve as predictive biomarkers of TACE efficacy and clinical outcomes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Gut microbiome remodeling across hepatocellular carcinoma progression and transarterial chemoembolization is associated with therapeutic response and prognosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月3日

查看全文
  • Bing Liu,
  • Shu-Hui Wan,
  • Yi-Tian Zhang,
  • Jun-Hao Lin,
  • Xun-Chang Ke,
  • Wei-Hua Lin,
  • Mei-Xiao Zhan

摘要

Clinical outcomes after transarterial chemoembolization (TACE) for intermediate–to–advanced hepatocellular carcinoma (HCC) are highly variable, and reliable predictive biomarkers are lacking. Although gut microbiota dysbiosis has been implicated in HCC pathogenesis and immunotherapy response, its role in TACE outcomes remains unclear. This study aimed to delineate gut microbiome remodeling across HCC progression and TACE treatment and to evaluate its association with clinical outcomes. Fecal samples were collected from healthy controls (n = 10), newly diagnosed untreated HCC patients (n = 30), post-TACE HCC patients (n = 29), and a longitudinal cohort of TACE-treated patients (n = 56). Gut microbiota profiles were analyzed using 16S rDNA sequencing. Differential taxa, microbial diversity, functional pathways, and survival associations were assessed using bioinformatics and Kaplan–Meier analyses. Gut microbiome composition differed markedly across healthy controls, untreated HCC, and post-TACE patients. Bacteroides abundance progressively decreased, whereas Prevotella_9 increased across disease progression and treatment. Among TACE-treated patients, progressive disease was characterized by higher Prevotella_9 and lower Bacteroides and Ruminococcus abundance, accompanied by significantly reduced microbial diversity. Kaplan–Meier analysis showed that low Prevotella_9 (P = 0.001) and high Bacteroides (P = 0.001) and Faecalibacterium (P = 0.04) were associated with prolonged progression-free survival, while higher Phascolarctobacterium and Veillonella abundance correlated with shorter overall survival. Functional prediction revealed alterations in pathways related to lipid metabolism, carbohydrate metabolism, and immune regulation. Gut microbiome remodeling is closely associated with HCC progression and TACE outcomes. Specific microbial taxa, particularly Bacteroides and Prevotella_9, were associated with therapeutic response and prognosis; however, these findings are exploratory and warrant We hypothesized that specific microbial taxa and metabolic pathways could serve as predictive biomarkers of TACE efficacy and clinical outcomes.