<p>Some conventional structural indices (e.g., Shannon–Wiener, average taxonomic diversity (∆), and AZTI´s Marine Biotic Index (AMBI)) and functional indicators (such as BPc and BTA) were examined on macrobenthic communities in the Gorgan Bay. For assessing the current conditions of the entrance environment of the Gorgan Bay, macrobenthic communities data were collected monthly over a 1-year period in 2019 from sites with different anthropogenic impacts including the discharge of the coastal cities basin, cage culture of sturgeons, tourism, and Qarasu river estuary. The results indicated a declining trend in the structure and functionality of Gorgan Bay, in which macrobenthic communities responded to disturbances such as bed changes, increases in organic matter, and impacts on some environmental factors, including a reduction in dissolved oxygen and relative increase in the salinity alongside the slope of the Gorgan Bay entrance. Biological traits analysis showed that the lowest changes in macrobenthic density and biomass were observed in north and middle stations of the Gorgan Bay in which human activities were low. In addition, almost all structural and functional indices demonstrated dominant environmental conditions (except for Pielou’s index and average taxonomic diversity). This study would also provide strong baseline research for monitoring future management actions in Gorgan Bay. Considering different aspects of the functional diversity of macrobenthic communities can assist in illuminating the processes and mechanisms through which ecosystems recover following restoration. We encourage the use of trait-based methods to detect trends in methods and patterns that can guide future bay restoration plans.</p>