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<p>Phage therapy offers a promising alternative to antibiotics, with lysin capacity being a key determinant of therapeutic efficacy. This study isolated two <i>Enterococcus faecalis</i> phages, vB_EfaS_785CC and vB_EfaS_785CS, from aquatic market sewage. They are classified as species LY0322 in the genus <i>Efquatrovirus</i> of the class <i>Caudoviricetes</i>, had rapid lysis and potent antibacterial activity against multidrug-resistant enterococci, highlighting their biocontrol potential. Despite a 99.95% nucleotide sequence similarity, these two phages display significant phenotypic differences in lysis ability. Comparative genomic analysis indicates that these lytic differences stem primarily from variable-length homopolymer-tracts in their genomes, which induce coding mutations affecting tail proteins and DNA metabolism genes, thus influencing lysis capacity. The findings guide future research in enhancing phage lytic potential.</p>

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Isolation and characterization of Enterococcus phages vB_EfaS_785CC and vB_EfaS_785CS

  • 期刊论文

  • 发表日期：

    2026年6月19日

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  • Shixuan Huang,
  • Yangkai Li,
  • Zekun Liu,
  • Meiyan Yang

摘要

Phage therapy offers a promising alternative to antibiotics, with lysin capacity being a key determinant of therapeutic efficacy. This study isolated two Enterococcus faecalis phages, vB_EfaS_785CC and vB_EfaS_785CS, from aquatic market sewage. They are classified as species LY0322 in the genus Efquatrovirus of the class Caudoviricetes, had rapid lysis and potent antibacterial activity against multidrug-resistant enterococci, highlighting their biocontrol potential. Despite a 99.95% nucleotide sequence similarity, these two phages display significant phenotypic differences in lysis ability. Comparative genomic analysis indicates that these lytic differences stem primarily from variable-length homopolymer-tracts in their genomes, which induce coding mutations affecting tail proteins and DNA metabolism genes, thus influencing lysis capacity. The findings guide future research in enhancing phage lytic potential.