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<p>Low-temperature stress severely restricts plant growth and agricultural production. The INDUCER OF CBF EXPRESSION (ICE) transcription factors are key regulators of the plant cold response. To investigate the molecular characteristics of the ICE gene family and their regulatory functions in response to low-temperature stress in <i>Taraxacum kok-saghyz</i> (TKS), we systematically identified 7 <i>TksICE</i>s in TKS at the whole-genome level, and simultaneously selected 3 <i>HbICE</i>s in <i>Hevea brasiliensis</i> for comparative analysis. Results showed that the <i>ICE</i> gene family mainly expanded via whole-genome duplication and segmental duplication, evolved under purifying selection, and exhibited both evolutionary conservation and lineage-specific divergence. The promoters of <i>TksICE</i>s contained significantly more stress and hormone-responsive elements than those of <i>HbICEs</i>. Expression analysis revealed that <i>TksICE</i>s displayed tissue specificity, and most were upregulated under cold stress and cold acclimation. Subcellular localization and transcriptional activation assays confirmed that <i>TksICE6</i> was localized in the nucleus and exhibited obvious transcriptional activation activity. Yeast two-hybrid assays verified the physical interaction of TksICE6 with TksHOS1 and TksMUTE, suggesting its potential roles in cold signal transduction and stomatal development regulation. This study systematically characterized the structural features, evolutionary patterns of the <i>ICE</i> gene family in TKS and <i>H. brasiliensis</i>, as well as the expression profiles of <i>TksICE</i>s, providing a theoretical basis and candidate gene resources for elucidating the molecular mechanisms of cold tolerance in TKS and facilitating molecular breeding.</p>

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Genome-wide identification and cold stress expression of inducer of CBF expression (ICE) transcription factors in Taraxacum kok-saghyz

  • 期刊论文

  • 发表日期：

    2026年5月29日

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  • Shaoli Tan,
  • Jinxian Chen,
  • Zhu Wen,
  • Xueping Wu,
  • Shuangxian Liu,
  • Haixia Wu,
  • Fuyuan Jin,
  • Xiaochang Su,
  • Shuzhen Wu,
  • Yujun Xu,
  • Shugang Hui,
  • Wei Li,
  • Xuchu Wang

摘要

Low-temperature stress severely restricts plant growth and agricultural production. The INDUCER OF CBF EXPRESSION (ICE) transcription factors are key regulators of the plant cold response. To investigate the molecular characteristics of the ICE gene family and their regulatory functions in response to low-temperature stress in Taraxacum kok-saghyz (TKS), we systematically identified 7 TksICEs in TKS at the whole-genome level, and simultaneously selected 3 HbICEs in Hevea brasiliensis for comparative analysis. Results showed that the ICE gene family mainly expanded via whole-genome duplication and segmental duplication, evolved under purifying selection, and exhibited both evolutionary conservation and lineage-specific divergence. The promoters of TksICEs contained significantly more stress and hormone-responsive elements than those of HbICEs. Expression analysis revealed that TksICEs displayed tissue specificity, and most were upregulated under cold stress and cold acclimation. Subcellular localization and transcriptional activation assays confirmed that TksICE6 was localized in the nucleus and exhibited obvious transcriptional activation activity. Yeast two-hybrid assays verified the physical interaction of TksICE6 with TksHOS1 and TksMUTE, suggesting its potential roles in cold signal transduction and stomatal development regulation. This study systematically characterized the structural features, evolutionary patterns of the ICE gene family in TKS and H. brasiliensis, as well as the expression profiles of TksICEs, providing a theoretical basis and candidate gene resources for elucidating the molecular mechanisms of cold tolerance in TKS and facilitating molecular breeding.