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<p>The β-D-xylosidase (BXL) gene family, belonging to the glycoside hydrolase GH3 family, plays a crucial role in cell wall remodeling and plant defense. However, its genomic characterization and functional roles in potato (<i>Solanum tuberosum</i>) remain unexplored. In this study, seven StBXL genes (StBXL1–StBXL7) were systematically identified from the potato genome, all harboring conserved Glyco_hydro_3 and Glyco_hydro_3_C domains, indicating evolutionary conservation comparable to Arabidopsis. Promoter analysis revealed enrichment of hormone-responsive (e.g., MeJA-related CGTCA/TGACG motifs), abiotic stress-related, and growth-regulatory cis-elements, suggesting integration of hormonal and environmental signals in defense regulation. Expression profiling demonstrated that <i>Phytophthora infestans</i> infection significantly induced <i>StBXL4</i> expression. Functional assays in <i>Nicotiana benthamiana</i> showed that <i>StBXL4</i> overexpression enhanced resistance to <i>P. infestans</i> by upregulating salicylic acid (SA)- and jasmonic acid (JA)-responsive genes (<i>NbPR1A</i>, <i>NbPR2</i>, <i>NbPR5</i>, <i>NbICS1</i>, <i>NbMYC2</i>), pattern-triggered immunity (PTI) marker genes (<i>WRKY7</i>, <i>WRKY8</i>), and antioxidant enzymes (<i>POD</i>), thereby reducing excessive H₂O₂ accumulation. Conversely, silencing of <i>NbBXL4</i> increased disease susceptibility and impaired ROS scavenging capacity. These findings reveal that <i>StBXL4</i> acts as a dual-function regulator in potato immunity by maintaining ROS homeostasis and coordinating multiple defense signaling pathways. This study not only fills the genomic gap of the potato BXL family but also identifies <i>StBXL4</i> as a promising candidate for molecular breeding strategies to enhance durable late blight resistance.</p>

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Genome-wide identification of the BXL gene family in potato (Solanum tuberosum) and functional characterization of StBXL4 in disease resistance

  • 期刊论文

  • 发表日期：

    2026年2月13日

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  • Yuxin Li,
  • Yuhan Zhang,
  • Ying Shi,
  • Xia Zhu,
  • Jingye Fu,
  • Qiang Wang

摘要

The β-D-xylosidase (BXL) gene family, belonging to the glycoside hydrolase GH3 family, plays a crucial role in cell wall remodeling and plant defense. However, its genomic characterization and functional roles in potato (Solanum tuberosum) remain unexplored. In this study, seven StBXL genes (StBXL1–StBXL7) were systematically identified from the potato genome, all harboring conserved Glyco_hydro_3 and Glyco_hydro_3_C domains, indicating evolutionary conservation comparable to Arabidopsis. Promoter analysis revealed enrichment of hormone-responsive (e.g., MeJA-related CGTCA/TGACG motifs), abiotic stress-related, and growth-regulatory cis-elements, suggesting integration of hormonal and environmental signals in defense regulation. Expression profiling demonstrated that Phytophthora infestans infection significantly induced StBXL4 expression. Functional assays in Nicotiana benthamiana showed that StBXL4 overexpression enhanced resistance to P. infestans by upregulating salicylic acid (SA)- and jasmonic acid (JA)-responsive genes (NbPR1A, NbPR2, NbPR5, NbICS1, NbMYC2), pattern-triggered immunity (PTI) marker genes (WRKY7, WRKY8), and antioxidant enzymes (POD), thereby reducing excessive H₂O₂ accumulation. Conversely, silencing of NbBXL4 increased disease susceptibility and impaired ROS scavenging capacity. These findings reveal that StBXL4 acts as a dual-function regulator in potato immunity by maintaining ROS homeostasis and coordinating multiple defense signaling pathways. This study not only fills the genomic gap of the potato BXL family but also identifies StBXL4 as a promising candidate for molecular breeding strategies to enhance durable late blight resistance.