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<p>The TCP (Teosinte branched1/Cincinnata/Proliferating Cell Factor) gene family encodes plant-specific transcription factors that are essential for regulating various aspects of growth, development, and environmental stress responses. To elucidate the roles of TCP genes in regulating the development of mustard (<i>Brassica juncea</i>), this study conducted a genome-wide identification and comprehensive analysis of TCP family members. A total of 72 <i>TCP</i> genes were identified in the genome of leaf mustard, distributed unevenly across 17 chromosomes. Based on conserved domain characteristics, the <i>BjTCPs</i> were classified into two subfamilies. Cis-acting element analysis of the promoter regions revealed numerous elements associated with hormonal responses, light signaling, stress tolerance, and development. Expression pattern analysis demonstrated that <i>BjTCP</i> genes exhibit different expression profiles. Functional validation through CRISPR/Cas9-mediated gene editing and transformation experiments indicated that <i>BjTCP18</i> acts as a negative regulator in the branching development of mustard. This study provides a theoretical foundation and candidate gene resources for further elucidation of the influence of the TCP gene family on regulatory processes and the role of the <i>BjTCP18</i> gene in regulating branching development in mustard.</p>

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Genome-wide identification of TCP gene family and gene editing of BjTCP18 genes increases branching in Brassica juncea

  • 期刊论文

  • 发表日期：

    2025年11月12日

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  • Shuangping Heng,
  • Meng Li,
  • Rouyi Zhao,
  • Boning Cao,
  • Wei Zeng,
  • Guangzhi Mao,
  • Feng Xing,
  • Shuaibin Lian,
  • Shaoheng Zhang

摘要

The TCP (Teosinte branched1/Cincinnata/Proliferating Cell Factor) gene family encodes plant-specific transcription factors that are essential for regulating various aspects of growth, development, and environmental stress responses. To elucidate the roles of TCP genes in regulating the development of mustard (Brassica juncea), this study conducted a genome-wide identification and comprehensive analysis of TCP family members. A total of 72 TCP genes were identified in the genome of leaf mustard, distributed unevenly across 17 chromosomes. Based on conserved domain characteristics, the BjTCPs were classified into two subfamilies. Cis-acting element analysis of the promoter regions revealed numerous elements associated with hormonal responses, light signaling, stress tolerance, and development. Expression pattern analysis demonstrated that BjTCP genes exhibit different expression profiles. Functional validation through CRISPR/Cas9-mediated gene editing and transformation experiments indicated that BjTCP18 acts as a negative regulator in the branching development of mustard. This study provides a theoretical foundation and candidate gene resources for further elucidation of the influence of the TCP gene family on regulatory processes and the role of the BjTCP18 gene in regulating branching development in mustard.