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<p>Increasing environmental releases of vanadium (V) by industrial plants pose significant risks of soil, water, and ecosystem contamination. Microbes reduce this toxic compound by producing a vanadate reductase enzyme that converts V<sup>5+</sup> to a less toxic compound, V<sup>4+</sup>. This study describes the cloning, expression, and characterization of an asparaginyl-tRNA synthetase (AsnRS) from the marine bacterium <i>Shewanella</i> sp. HU-ET00281. The <i>Shewanella-</i>AsnRS (<i>Sh-</i>AsnRS) recombinant protein reduced V<sup>5+</sup> to V<sup>4+</sup> under ambient conditions. The K<sub>m</sub> and V<sub>max</sub> values were 8.70 mM and 4.45&#xa0;mol-V<sup>4+</sup>/min/mol-protein respectively. Activity was enhanced by up to 75% on addition of Zn<sup>2+</sup> and Cu<sup>2+</sup>. <i>Sh-</i>AsnRS is a novel dual-function enzyme with AsnRS and V<sup>5+</sup> reductase activities that will aid bioremediation and environmental detoxification.</p>

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Asparaginyl-tRNA Synthetase (AsnRS) Is a Novel Vanadate Reductase from a Marine Bacterium

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月23日

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  • Dewi Yuliani,
  • Romaidi,
  • Tatsuya Ueki

摘要

Increasing environmental releases of vanadium (V) by industrial plants pose significant risks of soil, water, and ecosystem contamination. Microbes reduce this toxic compound by producing a vanadate reductase enzyme that converts V5+ to a less toxic compound, V4+. This study describes the cloning, expression, and characterization of an asparaginyl-tRNA synthetase (AsnRS) from the marine bacterium Shewanella sp. HU-ET00281. The Shewanella-AsnRS (Sh-AsnRS) recombinant protein reduced V5+ to V4+ under ambient conditions. The Km and Vmax values were 8.70 mM and 4.45 mol-V4+/min/mol-protein respectively. Activity was enhanced by up to 75% on addition of Zn2+ and Cu2+. Sh-AsnRS is a novel dual-function enzyme with AsnRS and V5+ reductase activities that will aid bioremediation and environmental detoxification.