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<p>To identify sex-biased gene and miRNA expression during the critical period of sex differentiation in the prawn <i>Macrobrachium rosenbergii</i>, we performed whole-body RNA sequencing of males and females at post-larval days 40 (PL40) and 70 (PL70). We obtained a total of 18,244 known and 7,063 novel mRNAs, along with 1,474 known and 1,049 novel miRNAs. A differential expression analysis revealed 1,323 sex-biased genes and 34 sex-biased miRNAs as well as seven miRNA-mRNA co-expression pairs, including two pairs associated with sex differentiation. The top 30 GO terms and KEGG pathways associated with sex-biased loci at PL40 and PL70 were evaluated. Four genes and three miRNAs with potential roles in sex differentiation were identified. Notably, through dual-luciferase reporter gene assays in 293T cells, we verified that the miRNAs <i>let-7b</i> and <i>miR-9-5p</i> regulate the expression of the <i>dsx</i> gene. Our research provides new insight into the sex differentiation mechanisms in <i>M. rosenbergii</i>.</p>

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Whole-body RNA Sequencing Analysis of the Post-larval Reveals the Sex-biased Gene and MiRNA in the Prawn Macrobrachium rosenbergii

  • 期刊论文

  • 发表日期：

    2026年1月9日

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  • Kun Xie,
  • Yu-Meng Shi,
  • Jin-Xia Yang,
  • Jia-Yan Chen,
  • Xu Huang,
  • Xin-Yu Liu,
  • Li-Ping Chen,
  • Jie Chen,
  • Chao-Ping Lu,
  • Gao-Feng Qiu

摘要

To identify sex-biased gene and miRNA expression during the critical period of sex differentiation in the prawn Macrobrachium rosenbergii, we performed whole-body RNA sequencing of males and females at post-larval days 40 (PL40) and 70 (PL70). We obtained a total of 18,244 known and 7,063 novel mRNAs, along with 1,474 known and 1,049 novel miRNAs. A differential expression analysis revealed 1,323 sex-biased genes and 34 sex-biased miRNAs as well as seven miRNA-mRNA co-expression pairs, including two pairs associated with sex differentiation. The top 30 GO terms and KEGG pathways associated with sex-biased loci at PL40 and PL70 were evaluated. Four genes and three miRNAs with potential roles in sex differentiation were identified. Notably, through dual-luciferase reporter gene assays in 293T cells, we verified that the miRNAs let-7b and miR-9-5p regulate the expression of the dsx gene. Our research provides new insight into the sex differentiation mechanisms in M. rosenbergii.