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<p><i>Perkinsus olseni</i> is a highly pathogenic protozoan parasite affecting mollusks worldwide, posing a significant threat to Manila clam (<i>Ruditapes philippinarum</i>) aquaculture. This study investigated the impact of natural <i>P. olseni</i> infection abundance on the gill lipid metabolism of clams collected from Dandong City, Liaoning Province. The parasite species was identified via Polymerase Chain Reaction (PCR), while infection prevalence, abundance, and infection grade classification were assessed using Ray’s Fluid Thioglycolate Medium (RFTM) culture. Lipid metabolomics analysis was performed to compare lipid profiles among uninfected (Neg), lightly infected (L), and moderately infected (M) clam gills. Results confirmed the presence of <i>P. olseni</i> with an overall prevalence of 91%. The infection abundance was significantly higher in the gills and digestive gland than in the mantle and siphon tissues. Lipidomic analysis of 18 samples (6 biological replicates per Neg, L, and M group) using a Liquid Chromatography-Mass Spectrometry (LC-MS) platform identified 1,561 metabolites, with significant differences observed across infection groups. 28 differential lipid metabolites were common to both the L and M groups when compared to the Neg group. KEGG pathway enrichment and correlation network analysis highlighted two key metabolites: diacylglycerol (DG) (16:0/18:2) and triacylglycerol (TG) (18:0/18:1/18:2). These metabolites were associated with 13 pathways, including T cell receptor signaling, fat digestion and absorption, and regulation of lipolysis in adipocytes. These findings elucidate tissue-specific infection patterns and reveal lipid metabolic reprogramming in clam gills in response to <i>P. olseni</i>, providing insights into host-parasite interactions and potential metabolic biomarkers for perkinsosis.</p>

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Effect of Natural Perkinsus Olseni Infection Abundance on the Lipid Metabolism in the Gills of Manila Clam (Ruditapes philippinarum)

  • 期刊论文

  • 发表日期：

    2025年11月26日

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  • Haoyi Jiang,
  • Yuxiang Wang,
  • Shengli Fu,
  • Jie Lu,
  • Tuo Yao,
  • Jingyue Zhang,
  • Zhaozhong Zhang,
  • Yaotong Hao,
  • Zhifu Liang,
  • Lingtong Ye

摘要

Perkinsus olseni is a highly pathogenic protozoan parasite affecting mollusks worldwide, posing a significant threat to Manila clam (Ruditapes philippinarum) aquaculture. This study investigated the impact of natural P. olseni infection abundance on the gill lipid metabolism of clams collected from Dandong City, Liaoning Province. The parasite species was identified via Polymerase Chain Reaction (PCR), while infection prevalence, abundance, and infection grade classification were assessed using Ray’s Fluid Thioglycolate Medium (RFTM) culture. Lipid metabolomics analysis was performed to compare lipid profiles among uninfected (Neg), lightly infected (L), and moderately infected (M) clam gills. Results confirmed the presence of P. olseni with an overall prevalence of 91%. The infection abundance was significantly higher in the gills and digestive gland than in the mantle and siphon tissues. Lipidomic analysis of 18 samples (6 biological replicates per Neg, L, and M group) using a Liquid Chromatography-Mass Spectrometry (LC-MS) platform identified 1,561 metabolites, with significant differences observed across infection groups. 28 differential lipid metabolites were common to both the L and M groups when compared to the Neg group. KEGG pathway enrichment and correlation network analysis highlighted two key metabolites: diacylglycerol (DG) (16:0/18:2) and triacylglycerol (TG) (18:0/18:1/18:2). These metabolites were associated with 13 pathways, including T cell receptor signaling, fat digestion and absorption, and regulation of lipolysis in adipocytes. These findings elucidate tissue-specific infection patterns and reveal lipid metabolic reprogramming in clam gills in response to P. olseni, providing insights into host-parasite interactions and potential metabolic biomarkers for perkinsosis.