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<p><i>Penaeus japonicus</i> is a high-value crustacean species, but its response to oxygen nanobubble (NB-O<sub>2</sub>) remains poorly understood. This study evaluated the effects of NB-O<sub>2</sub> exposure on juvenile <i>P. japonicus</i> cultured in two recirculating aquaculture systems (RAS). The control group (LS) was maintained with low-saturation dissolved oxygen (DO) at 7.5 ± 0.5&#xa0;mg/L, while the experimental group (HS) was exposed to high-saturation DO of 15.0 ± 0.5&#xa0;mg/L using NB-O<sub>2</sub> technology. The investigation focused on changes in intestinal microbiota, transcriptomic responses, and physiological adaptation. Results demonstrated that NB-O<sub>2</sub> significantly improved growth performance metrics, including specific growth rate (SGR), feed conversion ratio (FCR), molting rate (MR), and survival rate (SR). The intestinal microbiota exhibited an increased abundance of beneficial microorganisms, such as Lactobacillales and <i>Rhodopseudomonas palustris</i>, while maintaining microbial stability. Transcriptome analysis identified key pathways associated with adaptation to hyperoxia, particularly those involved in amino acid metabolism, energy metabolism, and antioxidant capacity. These findings were supported by physiological analysis, which showed changes in the amino acid profile and total free amino acids (T-AA), as well as a balanced interaction between glycogen (Gn), adenosine triphosphatase (ATP), and lactate dehydrogenase (LDH) in energy regulation. Antioxidant enzyme activities were all elevated under hyperoxia, including peroxidase (POD), catalase (CAT), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px). Additionally, the expression of stress-resistance genes (<i>HSP70</i>, <i>CAP3</i>, <i>LYZ-C</i>) indicated no adverse effects. Overall, <i>P. japonicus</i> exhibited strong physiological and molecular adaptations to NB-O<sub>2</sub>-induced hyperoxia, highlighting the potential of this technology to enhance aquaculture productivity.</p> Graphical Abstract <p></p>

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Integrated Microbiome, Transcriptome, and Physiology Analyses Reveal the Response Of Kuruma Shrimp (Penaeus japonicus) to Oxygen Nanobubble Exposure

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Yi Chen,
  • Rongwei Zhang,
  • Yuntian Zhang,
  • Jiadong Guo,
  • Zhixu Guo,
  • Yuzhe Han,
  • Fuqiang Wang,
  • Xiaoran Zhao,
  • Tongjun Ren

摘要

Penaeus japonicus is a high-value crustacean species, but its response to oxygen nanobubble (NB-O2) remains poorly understood. This study evaluated the effects of NB-O2 exposure on juvenile P. japonicus cultured in two recirculating aquaculture systems (RAS). The control group (LS) was maintained with low-saturation dissolved oxygen (DO) at 7.5 ± 0.5 mg/L, while the experimental group (HS) was exposed to high-saturation DO of 15.0 ± 0.5 mg/L using NB-O2 technology. The investigation focused on changes in intestinal microbiota, transcriptomic responses, and physiological adaptation. Results demonstrated that NB-O2 significantly improved growth performance metrics, including specific growth rate (SGR), feed conversion ratio (FCR), molting rate (MR), and survival rate (SR). The intestinal microbiota exhibited an increased abundance of beneficial microorganisms, such as Lactobacillales and Rhodopseudomonas palustris, while maintaining microbial stability. Transcriptome analysis identified key pathways associated with adaptation to hyperoxia, particularly those involved in amino acid metabolism, energy metabolism, and antioxidant capacity. These findings were supported by physiological analysis, which showed changes in the amino acid profile and total free amino acids (T-AA), as well as a balanced interaction between glycogen (Gn), adenosine triphosphatase (ATP), and lactate dehydrogenase (LDH) in energy regulation. Antioxidant enzyme activities were all elevated under hyperoxia, including peroxidase (POD), catalase (CAT), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px). Additionally, the expression of stress-resistance genes (HSP70, CAP3, LYZ-C) indicated no adverse effects. Overall, P. japonicus exhibited strong physiological and molecular adaptations to NB-O2-induced hyperoxia, highlighting the potential of this technology to enhance aquaculture productivity.

Graphical Abstract