Scholarlink

<p>We found filamentous structures resembling conchocelis of red algae within the nacreous layer of the Akoya pearl oyster <i>Pinctada fucata</i> shell. Microscopic analyses revealed that these filamentous structures had an autofluorescence and varied in length, occasionally branched, and exhibited diameters ranging from 2 to 5&#xa0;μm—consistent with previously reported sizes for Bangiales conchocelis. We extracted DNA from organic matrix sheets obtained by decalcifying the shell and performed PCR with primers targeting red algae from the family Bangiales to identify these structures. Sequencing analysis confirmed a 94% match with that of <i>Bangia fuscopurpurea</i>, establishing the presence of <i>Bangia</i> conchocelis within the shell. Conchocelis distributed predominantly near the anterior ear across all examined shells. In 3-year-old oysters, conchocelis filament densities reached approximately 250 filaments per 1 mm<sup>2</sup>, with a broader distribution area than a 2-year-old oyster, where densities were lower, peaking at 35 filaments per 1 mm<sup>2</sup>. Conchocelis distribution was consistent between the left and right shells of the identical oyster. These findings suggest that conchocelis density and distribution expand with oyster growth, providing insights into the age-related dynamics of conchocelis localization in Akoya pearl oyster shells.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Distribution of Conchocelis of Red Algae Bangia sp. in the Nacreous Layer of Akoya Pearl Oyster Pinctada fucata Shell

  • 期刊论文

  • 发表日期：

    2025年4月16日

查看全文
  • Yukino Mizutani,
  • Naohisa Kato,
  • Misato Okamura,
  • Kiyohito Nagai,
  • Kaoru Maeyama,
  • Michio Suzuki,
  • Toshiyuki Shibata,
  • Makoto Kakinuma,
  • Daisuke Funabara

摘要

We found filamentous structures resembling conchocelis of red algae within the nacreous layer of the Akoya pearl oyster Pinctada fucata shell. Microscopic analyses revealed that these filamentous structures had an autofluorescence and varied in length, occasionally branched, and exhibited diameters ranging from 2 to 5 μm—consistent with previously reported sizes for Bangiales conchocelis. We extracted DNA from organic matrix sheets obtained by decalcifying the shell and performed PCR with primers targeting red algae from the family Bangiales to identify these structures. Sequencing analysis confirmed a 94% match with that of Bangia fuscopurpurea, establishing the presence of Bangia conchocelis within the shell. Conchocelis distributed predominantly near the anterior ear across all examined shells. In 3-year-old oysters, conchocelis filament densities reached approximately 250 filaments per 1 mm2, with a broader distribution area than a 2-year-old oyster, where densities were lower, peaking at 35 filaments per 1 mm2. Conchocelis distribution was consistent between the left and right shells of the identical oyster. These findings suggest that conchocelis density and distribution expand with oyster growth, providing insights into the age-related dynamics of conchocelis localization in Akoya pearl oyster shells.