Scholarlink

<p>Coastal beach environments harbor diverse bacterial communities, yet their antimicrobial potential remains largely unexplored. This study evaluates the antimicrobial potential of a bacterial isolate 2AT10 from Brazilian beach sand through <i>in vitro</i>, <i>in silico</i>, and <i>ex vivo</i> assays. Isolate 2AT10, a rod-shaped Gram-positive bacterium, exhibited broad-spectrum antimicrobial activity, with its culture supernatants showing greater efficacy against Gram-positive bacteria, including multidrug-resistant strains such as methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Antimicrobial activity from two differently processed supernatants decreased after heat treatment, enzymatic digestion, and pH changes, suggesting similar proteolytic profiles. Fourier-transform infrared spectroscopy (FTIR) revealed peaks corresponding to C = O and NO₂ groups, indicating the presence of amide functional groups. Importantly, the supernatants did not cause irritation in the&#xa0;<i>ex</i> <i>vivo</i> chorioallantoic membrane assay. Genomic analysis identified isolate 2AT10 as <i>Bacillus stercoris</i>, harboring genes encoding subtilosin A, bacilysin, bacillibactin, bacillaene and lactococcin-like bacteriocins, all associated with antimicrobial activity. A mutation in the sboA gene, involved in subtilosin A biosynthesis and previously linked to <i>in vitro</i> hemolytic activity and enhanced activity against Gram-positive bacteria, was also detected. Molecular docking and dynamics analyses suggested a possible interaction between lactococcin-like bacteriocin and a membrane component of the <i>S. aureus</i> phosphotransferase system transporter, possibly increasing membrane permeability.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-guided antimicrobial potential of Bacillus stercoris from coastal sand with activity against multidrug-resistant bacteria, including MRSA

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月1日

查看全文
  • Camilla Andrade Silva Valença,
  • Bruno Andrade,
  • Marcus Viana,
  • Ana Andrea Teixeira Barbosa,
  • Silvio Santana Dolabella,
  • Patrícia Severino,
  • Guillaume Ménard,
  • Yoann Augagneur,
  • Bertram Brenig,
  • Eliana B. Souto,
  • Vasco Azevedo,
  • Eric Guedon,
  • Sona Jain

摘要

Coastal beach environments harbor diverse bacterial communities, yet their antimicrobial potential remains largely unexplored. This study evaluates the antimicrobial potential of a bacterial isolate 2AT10 from Brazilian beach sand through in vitro, in silico, and ex vivo assays. Isolate 2AT10, a rod-shaped Gram-positive bacterium, exhibited broad-spectrum antimicrobial activity, with its culture supernatants showing greater efficacy against Gram-positive bacteria, including multidrug-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). Antimicrobial activity from two differently processed supernatants decreased after heat treatment, enzymatic digestion, and pH changes, suggesting similar proteolytic profiles. Fourier-transform infrared spectroscopy (FTIR) revealed peaks corresponding to C = O and NO₂ groups, indicating the presence of amide functional groups. Importantly, the supernatants did not cause irritation in the ex vivo chorioallantoic membrane assay. Genomic analysis identified isolate 2AT10 as Bacillus stercoris, harboring genes encoding subtilosin A, bacilysin, bacillibactin, bacillaene and lactococcin-like bacteriocins, all associated with antimicrobial activity. A mutation in the sboA gene, involved in subtilosin A biosynthesis and previously linked to in vitro hemolytic activity and enhanced activity against Gram-positive bacteria, was also detected. Molecular docking and dynamics analyses suggested a possible interaction between lactococcin-like bacteriocin and a membrane component of the S. aureus phosphotransferase system transporter, possibly increasing membrane permeability.

Graphical Abstract