Scholarlink

<p><i>Bacillus subtilis</i> BAGL, isolated from the rhizosphere, demonstrated significant antifungal activity against key phytopathogens, inhibiting <i>R. solani by 73.7%</i>,<i> F. oxysporum by 55.15%</i>,<i> and (A) solani by 77%</i>. Whole-genome sequencing revealed a genome size of 4,227,102&#xa0;bp, GC content of 43.08%, and 4,494 predicted genes. The accession number (PRJNA1349010) for the whole genome was obtained from the NCBI GenBank. Phylogenetic analysis confirmed BAGL’s taxonomic position of BAGL within the <i>B. subtilis</i> clade. The genome harbored 17 biosynthetic gene clusters (BGCs) responsible for producing secondary metabolites with antimicrobial properties, such as surfactin, fengycin, bacillibactin, and subtilosin (A) Additionally, BAGL carries a diverse set of carbohydrate-active enzymes (CAZymes) that may contribute to its biocontrol efficacy by disrupting fungal cell wall. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed BAGL’s involvement in multiple metabolic pathways, including secondary metabolite biosynthesis and carbon metabolism. Gene Ontology (GO) analysis highlighted numerous catalytic and binding activities crucial for metabolic and cellular processes. The presence of these genetic features suggests that <i>B. subtilis</i> BAGL is an effective candidate for developing eco-friendly disease management strategies in agricultural settings. Future investigations into the active compounds produced by BAGL and in vivo trials are essential to determine their efficacy in real-world settings.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole-genome sequencing and biosynthetic gene cluster analysis of Bacillus subtilis BAGL as a potent antifungal biocontrol agent against phytopathogenic fungi

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月13日

查看全文
  • Beenish Amjad,
  • Sana Sultan,
  • Asma Abro,
  • Bushra Tabassum,
  • Muhammad Ilyas,
  • Nusrat Jahan,
  • Samia Parveen,
  • Anwar Khan

摘要

Bacillus subtilis BAGL, isolated from the rhizosphere, demonstrated significant antifungal activity against key phytopathogens, inhibiting R. solani by 73.7%, F. oxysporum by 55.15%, and (A) solani by 77%. Whole-genome sequencing revealed a genome size of 4,227,102 bp, GC content of 43.08%, and 4,494 predicted genes. The accession number (PRJNA1349010) for the whole genome was obtained from the NCBI GenBank. Phylogenetic analysis confirmed BAGL’s taxonomic position of BAGL within the B. subtilis clade. The genome harbored 17 biosynthetic gene clusters (BGCs) responsible for producing secondary metabolites with antimicrobial properties, such as surfactin, fengycin, bacillibactin, and subtilosin (A) Additionally, BAGL carries a diverse set of carbohydrate-active enzymes (CAZymes) that may contribute to its biocontrol efficacy by disrupting fungal cell wall. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed BAGL’s involvement in multiple metabolic pathways, including secondary metabolite biosynthesis and carbon metabolism. Gene Ontology (GO) analysis highlighted numerous catalytic and binding activities crucial for metabolic and cellular processes. The presence of these genetic features suggests that B. subtilis BAGL is an effective candidate for developing eco-friendly disease management strategies in agricultural settings. Future investigations into the active compounds produced by BAGL and in vivo trials are essential to determine their efficacy in real-world settings.