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<p>Fusarium wilt of banana, caused by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 (<i>Foc</i> TR4), remains a severe global threat to banana production. This study aimed to identify and evaluate native fungal and actinomycete isolates from banana-growing regions in southern Vietnam for their potential as biological control agents against <i>Foc</i> TR4. Eighteen isolates were screened, and five antagonistic strains (<i>Trichoderma harzianum</i> D-LD, <i>T. asperelloides</i> G-BT, <i>Penicillium menonorum</i> LA02-2, <i>Streptomyces luteogriseus</i> XK3, and <i>S. mutabilis</i> XK4) were selected for morphological, molecular, enzymatic, and greenhouse evaluations. Both <i>T. harzianum</i> and <i>T. asperelloides</i> exhibited strong in vitro inhibition of <i>Foc</i> TR4 (87.4% and 84.2%, respectively) through the production of cell wall degrading enzymes, notably chitinase and protease. In greenhouse trials, <i>S. mutabilis</i> XK4 demonstrated the highest disease suppression (AUDPC reduction &gt; 80%, wilt incidence 28%) and enhanced plant growth, indicating a dual role in biocontrol and plant growth promotion. <i>Penicillium menonorum</i> LA02-2 showed moderate inhibition (50.9%) and growth-enhancing effects, highlighting its potential as a supplementary component in microbial consortia. Scanning electron microscopy confirmed mycoparasitic interaction between <i>T. asperelloides</i> G-BT and <i>Foc</i> TR4 hyphae, whereas <i>S. luteogriseus</i> XK3 and <i>S. mutabilis</i> XK4 caused severe hyphal damage through antibiosis and enzymatic lysis. This study reports the first isolation of <i>P. menonorum</i>,<i> S. luteogriseus</i>, and <i>S. mutabilis</i> from Vietnamese banana soils and identifies <i>S. mutabilis</i> XK4 as a promising candidate for developing bio-formulations against <i>Foc</i> TR4. These findings highlight the potential of indigenous microbial resources for sustainable Fusarium wilt management in Cavendish banana cultivation.</p>

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Biocontrol potential of a native fungi and actinomyces collection against Fusarium oxysporum f. sp. cubense tropical race 4 causing fusarium wilt disease on cavendish banana in Southern Vietnam

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Van T. Tran,
  • Toan Q. Dinh,
  • Don D. Le,
  • Trang T.P. Phan

摘要

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), remains a severe global threat to banana production. This study aimed to identify and evaluate native fungal and actinomycete isolates from banana-growing regions in southern Vietnam for their potential as biological control agents against Foc TR4. Eighteen isolates were screened, and five antagonistic strains (Trichoderma harzianum D-LD, T. asperelloides G-BT, Penicillium menonorum LA02-2, Streptomyces luteogriseus XK3, and S. mutabilis XK4) were selected for morphological, molecular, enzymatic, and greenhouse evaluations. Both T. harzianum and T. asperelloides exhibited strong in vitro inhibition of Foc TR4 (87.4% and 84.2%, respectively) through the production of cell wall degrading enzymes, notably chitinase and protease. In greenhouse trials, S. mutabilis XK4 demonstrated the highest disease suppression (AUDPC reduction > 80%, wilt incidence 28%) and enhanced plant growth, indicating a dual role in biocontrol and plant growth promotion. Penicillium menonorum LA02-2 showed moderate inhibition (50.9%) and growth-enhancing effects, highlighting its potential as a supplementary component in microbial consortia. Scanning electron microscopy confirmed mycoparasitic interaction between T. asperelloides G-BT and Foc TR4 hyphae, whereas S. luteogriseus XK3 and S. mutabilis XK4 caused severe hyphal damage through antibiosis and enzymatic lysis. This study reports the first isolation of P. menonorum, S. luteogriseus, and S. mutabilis from Vietnamese banana soils and identifies S. mutabilis XK4 as a promising candidate for developing bio-formulations against Foc TR4. These findings highlight the potential of indigenous microbial resources for sustainable Fusarium wilt management in Cavendish banana cultivation.