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<p>Alkaline pectinases are pivotal for sustainable industries such as textile scouring, juice clarification, and wastewater treatment; however, strains combining high catalytic activity, true alkalistability, and broad agro-industrial applicability remain scarce. Thirty pectinolytic isolates obtained from decayed fruits in Sidi Bel Abbes, Algeria, yielded three <i>Bacillus amyloliquefaciens</i> strains (SPR6, ST9, SC2) with superior enzyme productivity. Under optimized submerged fermentation (pH 9.0, 37&#xa0;°C, 48&#xa0;h), strain ST9 achieved 22.4 U/mL, a 1.78-fold increase over baseline, while replacing commercial pectin with orange peel enhanced yield by 1.32-fold and reduced substrate costs by 99%. The strains exhibited remarkable polyextremotolerance, maintaining viable growth and residual enzymatic activity under abiotic stress conditions (up to pH 12, 50&#xa0;°C, and 11% NaCl), beyond their optimal production range. Beyond pectinolysis, all isolates co-produced key hydrolases, expressed plant-growth-promoting (PGP) traits (indole-3-acetic acid, siderophores, phosphate solubilization), and suppressed <i>Fusarium</i> and <i>Aspergillus</i> spp. This first report of alkaline-adapted <i>B. amyloliquefaciens</i> producing industrially robust pectinase and exhibiting PGP potential highlights their promise as dual-purpose biocatalysts and bioinoculants for circular-bioeconomy applications.</p>

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Alkaline‑adapted pectinase from polyextremotolerant Bacillus amyloliquefaciens via agro-wastes valorization: dual biocatalyst and multifunctional bioinoculant

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Fatima Zohra Kaissar,
  • Mohamed Lamine Benine,
  • Sid Ahmed Saadi,
  • Amina Khaldi,
  • Amira Missouri,
  • Mohamed Laraki,
  • Sara Barberini,
  • Giovanni Emiliani,
  • Seif El Islam Lebouachera

摘要

Alkaline pectinases are pivotal for sustainable industries such as textile scouring, juice clarification, and wastewater treatment; however, strains combining high catalytic activity, true alkalistability, and broad agro-industrial applicability remain scarce. Thirty pectinolytic isolates obtained from decayed fruits in Sidi Bel Abbes, Algeria, yielded three Bacillus amyloliquefaciens strains (SPR6, ST9, SC2) with superior enzyme productivity. Under optimized submerged fermentation (pH 9.0, 37 °C, 48 h), strain ST9 achieved 22.4 U/mL, a 1.78-fold increase over baseline, while replacing commercial pectin with orange peel enhanced yield by 1.32-fold and reduced substrate costs by 99%. The strains exhibited remarkable polyextremotolerance, maintaining viable growth and residual enzymatic activity under abiotic stress conditions (up to pH 12, 50 °C, and 11% NaCl), beyond their optimal production range. Beyond pectinolysis, all isolates co-produced key hydrolases, expressed plant-growth-promoting (PGP) traits (indole-3-acetic acid, siderophores, phosphate solubilization), and suppressed Fusarium and Aspergillus spp. This first report of alkaline-adapted B. amyloliquefaciens producing industrially robust pectinase and exhibiting PGP potential highlights their promise as dual-purpose biocatalysts and bioinoculants for circular-bioeconomy applications.