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Purpose <p>This study optimizes keratinase production from chicken-feather waste by <i>Pseudomonas aeruginosa</i> PA1045 and <i>Alcaligenes faecalis</i> IHB B 6507, characterize and apply their hydrolysates for organic fertilizer application.</p> Methods <p>Keratinolytic activities of <i>P. aeruginosa</i> PA1045 and <i>A. faecalis</i> IHB B 6507 were assessed on chicken feather media. Factors optimized were temperature, pH, substrate concentration and inoculum size. Chicken-feather hydrolysate (CFH) was characterized via Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), protein and amino acid profiling using Bradford method and High-Performance Liquid Chromatography (HPLC) respectively. Following the application of CFH on maize plant, nitrogen content, phytochemical screening, chlorophyll concentration and plant growth parameters were assessed.</p> Result <p>Keratinolytic activity from <i>P. aeruginosa</i> peaked (42.48 U/mL) at day 5, while <i>A. faecalis</i> was maximum (40.88 U/mL) at day 7. Optimal keratinase production conditions by <i>P. aeruginosa</i> and <i>A. faecalis</i> were temperature 35&#xa0;°C, pH 8.0, 1.5% substrate concentration and inoculum size of 2 mL and 3 mL, respectively. SEM analysis confirmed feather degradation and loss of structural integrity in hydrolysates (<i>P. aeruginosa</i>– PaH; <i>A. faecalis</i>– AfH) compared to the organized control, while FTIR analysis revealed distinct peaks indicating compositional differences. The PaH demonstrated higher protein (7.46&#xa0;mg/100&#xa0;g) and nitrogen (6.688 ± 1.08%) content. L-Threonine was the most dominant amino acid in both hydrolysates. Significantly, plants treated with PaH and AfH displayed enhanced growth compared to NPK fertilizer and water controls.</p> Conclusion <p><i>P. aeruginosa</i> PA1045 and <i>A. faecalis</i> IHB B 6507 efficiently hydrolyzed chicken feathers under optimal conditions, yielding hydrolysates rich in essential amino acids, underscoring their multifaceted biotechnological applications in feather waste valorization and potential agricultural use.</p>

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Bioconversion of chicken feather waste: optimizing keratinase production by Pseudomonas aeruginosa PA1045 and Alcaligenes faecalis IHB B 6507, characterization and application as organic fertilizers

  • 期刊论文

  • 发表日期：

    2025年11月19日

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  • Frank Olakunle Otuyelu,
  • Patricia Folakemi Omojasola,
  • Olusoji Olusegun Adebisi,
  • Jonathan Maiangwa,
  • Ridwan Tope Azeez,
  • Aminat Alolade Ali,
  • Barakat Oluwabukunola Saheed,
  • Muinat Olanike Kazeem

摘要

Purpose

This study optimizes keratinase production from chicken-feather waste by Pseudomonas aeruginosa PA1045 and Alcaligenes faecalis IHB B 6507, characterize and apply their hydrolysates for organic fertilizer application.

Methods

Keratinolytic activities of P. aeruginosa PA1045 and A. faecalis IHB B 6507 were assessed on chicken feather media. Factors optimized were temperature, pH, substrate concentration and inoculum size. Chicken-feather hydrolysate (CFH) was characterized via Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), protein and amino acid profiling using Bradford method and High-Performance Liquid Chromatography (HPLC) respectively. Following the application of CFH on maize plant, nitrogen content, phytochemical screening, chlorophyll concentration and plant growth parameters were assessed.

Result

Keratinolytic activity from P. aeruginosa peaked (42.48 U/mL) at day 5, while A. faecalis was maximum (40.88 U/mL) at day 7. Optimal keratinase production conditions by P. aeruginosa and A. faecalis were temperature 35 °C, pH 8.0, 1.5% substrate concentration and inoculum size of 2 mL and 3 mL, respectively. SEM analysis confirmed feather degradation and loss of structural integrity in hydrolysates (P. aeruginosa– PaH; A. faecalis– AfH) compared to the organized control, while FTIR analysis revealed distinct peaks indicating compositional differences. The PaH demonstrated higher protein (7.46 mg/100 g) and nitrogen (6.688 ± 1.08%) content. L-Threonine was the most dominant amino acid in both hydrolysates. Significantly, plants treated with PaH and AfH displayed enhanced growth compared to NPK fertilizer and water controls.

Conclusion

P. aeruginosa PA1045 and A. faecalis IHB B 6507 efficiently hydrolyzed chicken feathers under optimal conditions, yielding hydrolysates rich in essential amino acids, underscoring their multifaceted biotechnological applications in feather waste valorization and potential agricultural use.