Scholarlink

<p>Antibiotic resistance poses a major threat to global health. This study focuses on <i>Streptomyces</i>, a genus of <i>Actinobacteria</i> known for antibiotic production. We aimed to investigate the antimicrobial activity and metabolic profile of <i>Streptomyces</i> sp. strains isolated from the unexplored regions of Khouribga province, Morocco, to discover new potential treatments. Forty isolates of <i>Actinobacteria</i> were subjected to a preliminary antimicrobial screening, using double-layer and cross-dragging methods against a variety of microorganisms. The most active isolates were characterized by various techniques, followed by fermentation and extraction with organic solvents. The antimicrobial activity of the extracts obtained was then assessed by disk diffusion against multidrug-resistant (MDR) bacteria and phytopathogenic fungi. The isolate E4-10 showed promising antimicrobial activity against MDR strains such as <i>E. coli</i> 23I2341, <i>Enterococcus</i> 23I2357, <i>S. aureus</i> 23K1625, and <i>S. saprophyticus</i> 23I2352, as well as phytopathogenic fungi like <i>Aspergillus niger</i>, <i>Penicillium</i> sp., and <i>C. albicans</i> ATCC 60193. GC–MS analysis revealed 18 bioactive compounds, including 2 major components: S-Methyl methanethiosulfonate (15.41%), and 5-oxopyrrolidine-3-carboxylic acid (21.44%). Furthermore, a computational study was investigated (Density Functional Theory (DFT), ADMET, and molecular docking) to analyze the 2 compounds, the results show that the chosen compounds possess promising structural and reactive properties, effectively interacting with proteins in <i>S. aureus</i>, <i>E. coli</i>, and <i>Fusarium</i> sp. Their binding to specific proteins affects membrane fluidity and permeability, while their compliance with pharmacokinetic criteria underscores their therapeutic potential as candidates for further research in treating bacterial and fungal infection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Computational approaches to the in vitro antimicrobial potential of Streptomyces paradoxus E4-10 extract against multidrug-resistant bacteria and phytopathogenic fungi

  • 期刊论文

  • 发表日期：

    2025年11月19日

查看全文
  • Said Rammali,
  • Achraf Abdou,
  • Zakaria Benchama,
  • Mohamed El Aalaoui,
  • Abdellatif Rahim,
  • Fatima Zahra Kamal,
  • Lhoussain Hajji,
  • Faouzia Benhallam,
  • Mohamed Dakir,
  • Bouchaib Bencharki

摘要

Antibiotic resistance poses a major threat to global health. This study focuses on Streptomyces, a genus of Actinobacteria known for antibiotic production. We aimed to investigate the antimicrobial activity and metabolic profile of Streptomyces sp. strains isolated from the unexplored regions of Khouribga province, Morocco, to discover new potential treatments. Forty isolates of Actinobacteria were subjected to a preliminary antimicrobial screening, using double-layer and cross-dragging methods against a variety of microorganisms. The most active isolates were characterized by various techniques, followed by fermentation and extraction with organic solvents. The antimicrobial activity of the extracts obtained was then assessed by disk diffusion against multidrug-resistant (MDR) bacteria and phytopathogenic fungi. The isolate E4-10 showed promising antimicrobial activity against MDR strains such as E. coli 23I2341, Enterococcus 23I2357, S. aureus 23K1625, and S. saprophyticus 23I2352, as well as phytopathogenic fungi like Aspergillus niger, Penicillium sp., and C. albicans ATCC 60193. GC–MS analysis revealed 18 bioactive compounds, including 2 major components: S-Methyl methanethiosulfonate (15.41%), and 5-oxopyrrolidine-3-carboxylic acid (21.44%). Furthermore, a computational study was investigated (Density Functional Theory (DFT), ADMET, and molecular docking) to analyze the 2 compounds, the results show that the chosen compounds possess promising structural and reactive properties, effectively interacting with proteins in S. aureus, E. coli, and Fusarium sp. Their binding to specific proteins affects membrane fluidity and permeability, while their compliance with pharmacokinetic criteria underscores their therapeutic potential as candidates for further research in treating bacterial and fungal infection.