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<p>Optimizing the production of bioemulsifiers is critical for sustainable industrial applications. In this study, 27 strains were isolated from activated sludge, and strain Y-1 was identified as the most promising candidate based on emulsification index (EI) and emulsification rating. Strain Y-1 exhibited excellent emulsifying and dispersing activities toward both liquid paraffin and crude oil, with an EI of 65%. Morphological characterization, physiological and biochemical tests, and 16S rDNA sequencing identified Y-1 as a member of the genus <i>Acinetobacter</i>. Fermentation conditions were optimized using single-factor experiments and response surface methodology, and the optimal conditions were determined to be a carbon-to-nitrogen (C/N) ratio of 1:1, inoculum concentration of 2%, and incubation temperature of 34&#xa0;°C. Under these conditions, the bioemulsifier yield of <i>Acinetobacter</i> Y-1 reached 6.9&#xa0;g/L, representing a 4.14-fold increase compared to unoptimized conditions. The bioemulsifier produced by <i>Acinetobacter</i> Y-1 effectively emulsified a variety of hydrocarbon substrates, including liquid paraffin, diesel, xylene, olive oil, cutting oil, cold rolling oil, n-hexane, and benzene. Furthermore, it retained stable emulsification activity under extreme conditions, including a wide temperature range (4–80&#xa0;°C), high salinity (5–30%), and broad pH values (1–14). These findings highlight <i>Acinetobacter</i> Y-1 as a promising strain for the production of high-activity, stable bioemulsifiers with great potential for industrial applications.</p>

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Screening, isolation, and process optimization of a bioemulsifier-producing Acinetobacter Y-1

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • Hui Wei,
  • Fu-Rong Yao,
  • Ou-Ying Li,
  • Shuo Guo,
  • Jun Li,
  • He-Jing Yan,
  • Feng-Mei Zhu,
  • Jie-Fang Zhou

摘要

Optimizing the production of bioemulsifiers is critical for sustainable industrial applications. In this study, 27 strains were isolated from activated sludge, and strain Y-1 was identified as the most promising candidate based on emulsification index (EI) and emulsification rating. Strain Y-1 exhibited excellent emulsifying and dispersing activities toward both liquid paraffin and crude oil, with an EI of 65%. Morphological characterization, physiological and biochemical tests, and 16S rDNA sequencing identified Y-1 as a member of the genus Acinetobacter. Fermentation conditions were optimized using single-factor experiments and response surface methodology, and the optimal conditions were determined to be a carbon-to-nitrogen (C/N) ratio of 1:1, inoculum concentration of 2%, and incubation temperature of 34 °C. Under these conditions, the bioemulsifier yield of Acinetobacter Y-1 reached 6.9 g/L, representing a 4.14-fold increase compared to unoptimized conditions. The bioemulsifier produced by Acinetobacter Y-1 effectively emulsified a variety of hydrocarbon substrates, including liquid paraffin, diesel, xylene, olive oil, cutting oil, cold rolling oil, n-hexane, and benzene. Furthermore, it retained stable emulsification activity under extreme conditions, including a wide temperature range (4–80 °C), high salinity (5–30%), and broad pH values (1–14). These findings highlight Acinetobacter Y-1 as a promising strain for the production of high-activity, stable bioemulsifiers with great potential for industrial applications.