<p>To investigate the efficiency of 450 nm blue light irradiation on bacterial inactivation in apheresis platelets and the effects of irradiation on the quality of apheresis platelets.&#xa0;The toxicity of 450 nm blue light on the Staphylococcus aureus (<i>S. aureus</i>) (10² CFU/ml). The bacterial cells were incubated with different time of 29 mW/cm² intensity 450 nm blue light (0, 0.5, 1, 2, 3, 4 h). Viable bacteria counts and platelet indices (platelet count, MPV, PDW, MA, PF) were measured pre- and post-irradiation. Proteomics and metabolomics analyses assessed changes in platelets and the impact of blue light.&#xa0;The numbers of viable bacteria in platelet decreased significantly after irradiation (<i>P</i> &lt; 0.05). No significant difference was detected in the physicochemical indexes of platelet (platelet counts, MPV, PDW, MA, PF) between the control group and the treated group (after irradiation) (<i>P</i> &gt; 0.05). Our platelet metabolomics analysis revealed alterations in 34 out of 981 quantified metabolites (3.5%). Additionally, we identified a total of 3726 proteins, and through comparative screening between two groups, we identified 71 differentially expressed proteins (1.9%), comprising 43 upregulated and 28 downregulated proteins. These findings suggest that a minimal number of proteins and metabolites exhibited changes in expression in the light-treated platelets compared to the untreated controls.&#xa0;The 450&#xa0;nm blue light of a certain intensity irradiation could sufficiently kill <i>S. aureus</i>, but had no significant effect on the quality of apheresis platelets. This research has provided further evidence supporting the potential for 450&#xa0;nm blue light to be developed as a pathogen reduction tool for stored platelets.</p>