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<p>This study aims to assess the effectiveness of Antimicrobial Photodynamic Therapy (aPDT) using phenothiazine photosensitizer (PS) combined with gold nanoparticles (DMMB-AuNPs) in treating <i>S. aureus</i>-contaminated wounds on the dorsum of rats. Twelve <i>Wistar</i> rats were divided into four groups: Control, LED, DMMB-AuNPs, and DMMB-AuNPs + LED. DMMB-AuNPs were prepared using DMMB (300 ng/mL) and 5&#xa0;nm diameter AuNPs (~ 5.5E + 13 particles/mL). The light source was a red LED (12&#xa0;J/cm<sup>2</sup>, λ630 ± 20&#xa0;nm, CW, 125 mW, 192s). Following the procedures, samples were plated on an agar medium specific to <i>S. aureus</i> to count the colony-forming units (CFU/mL). Statistical analysis using unidirectional ANOVA and Tukey’s test revealed that the aPDT group (DMMB-AuNPs + LED) exhibited a 99.999% (5 log reduction) microbial reduction with a significant statistical difference (<i>p</i> &lt; 0.0001) compared to the Control group, demonstrating a bactericidal effect. The in vivo findings unequivocally demonstrate the effectiveness of DMMB-AuNPs-mediated aPDT as an alternative therapy for staphylococcal infections.</p> Graphical abstract <p></p>

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Antimicrobial photodynamic therapy using gold nanoparticles conjugated with 1,9-dimethyl-methylene blue for treating Staphylococcus skin infections

  • 期刊论文

  • 发表日期：

    2025年2月19日

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  • Anildo Alves de Brito Júnior,
  • Pedro Jorge Louro Crugeira,
  • Wellington Luis Reis Costa,
  • Andressa Vollono Barbosa,
  • Susana Carla Pires Sampaio de Oliveira,
  • Maria Cristina Teixeira Cangussu,
  • Jean Nunes dos Santos,
  • Juliana Monteiro Azevedo,
  • Antonio Luiz Barbosa Pinheiro

摘要

This study aims to assess the effectiveness of Antimicrobial Photodynamic Therapy (aPDT) using phenothiazine photosensitizer (PS) combined with gold nanoparticles (DMMB-AuNPs) in treating S. aureus-contaminated wounds on the dorsum of rats. Twelve Wistar rats were divided into four groups: Control, LED, DMMB-AuNPs, and DMMB-AuNPs + LED. DMMB-AuNPs were prepared using DMMB (300 ng/mL) and 5 nm diameter AuNPs (~ 5.5E + 13 particles/mL). The light source was a red LED (12 J/cm2, λ630 ± 20 nm, CW, 125 mW, 192s). Following the procedures, samples were plated on an agar medium specific to S. aureus to count the colony-forming units (CFU/mL). Statistical analysis using unidirectional ANOVA and Tukey’s test revealed that the aPDT group (DMMB-AuNPs + LED) exhibited a 99.999% (5 log reduction) microbial reduction with a significant statistical difference (p < 0.0001) compared to the Control group, demonstrating a bactericidal effect. The in vivo findings unequivocally demonstrate the effectiveness of DMMB-AuNPs-mediated aPDT as an alternative therapy for staphylococcal infections.

Graphical abstract